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DAB (tetrahydrochloride hydrate) is commonly utilized as a chromogenic substrate in immunohistochemistry and enzyme histochemistry to reveal specific antigens or enzymes. Upon oxidation by hydrogen peroxide, DAB (tetrahydrochloride hydrate) forms a brown precipitate at the location of the antigen or enzyme, facilitating microscopic detection and observation. Additionally, DAB (tetrahydrochloride hydrate) holds potential applications in various research fields, such as the development of biosensors and the study of cellular oxidative stress.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
| Description | DAB (tetrahydrochloride hydrate) is commonly utilized as a chromogenic substrate in immunohistochemistry and enzyme histochemistry to reveal specific antigens or enzymes. Upon oxidation by hydrogen peroxide, DAB (tetrahydrochloride hydrate) forms a brown precipitate at the location of the antigen or enzyme, facilitating microscopic detection and observation. Additionally, DAB (tetrahydrochloride hydrate) holds potential applications in various research fields, such as the development of biosensors and the study of cellular oxidative stress. |
| Synonyms | 3,3'-Diaminobenzidine tetrahydrochloride hydrate |
| Molecular Weight | 380.14 |
| Formula | C12H22Cl4N4O |
| Cas No. | 868272-85-9 |
| Smiles | Cl.O.NC=1C=CC(=CC1N)C2=CC=C(N)C(N)=C2 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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