BODIPY-FL (BDP FL acid) is a broad-spectrum and effective fluorescent dye that can be used to label probes or primers and is a compound for the quantitative detection of specific DNA/RNA based on fluorescence bursts.BODIPY-FL-labelled monoterpenes can be used to detect Gram-positive and Gram-negative bacteria as characteristic and pathogenic fungi.

I. DNA/RNA detection:
1. Primer labeling: BODIPY-FL is chemically labeled with the target DNA primer or RNA probe. After labeling, the primer can be used for real-time PCR or other nucleic acid detection methods.
2. Fluorescence detection: The fluorescence signal changes of BODIPY-FL primers or probes are observed by real-time PCR instruments or fluorescence detectors, and then the quantitative analysis of DNA/RNA is performed.
3. Quantitative analysis: The content of target DNA/RNA is quantified according to the intensity of the fluorescence signal and the reaction curve.
II. Bacterial detection
1. Bacterial staining: BODIPY-FL dye is added to the bacterial sample and incubated at 37°C for 30 minutes to 1 hour.
2. Staining observation: BODIPY-FL-labeled bacteria are observed using a fluorescence microscope or flow cytometer, and real-time fluorescence imaging is performed to analyze the bacterial species and their number.
3. Identification of bacteria: Gram-positive and negative bacteria are identified based on the fluorescence characteristics of bacteria (such as color and brightness).
3. Fungal Detection
1. Fungal labeling: BODIPY-FL and fungal samples are stained and labeled by flow cytometry.
2. Microscopic observation: Fluorescent signals in samples are observed using a fluorescence microscope to confirm the fungal species.