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DAF-FM DA

Name: DAF-FM DA
Brand: TargetMol
SKU: T18965
Rating: 4.5 (1 reviews)

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Catalog No. T18965Cas No. 254109-22-3

Alias Diaminofluorescein-FM diacetate

DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm).

DAF-FM DA

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Catalog No. T18965Alias Diaminofluorescein-FM diacetateCas No. 254109-22-3

DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm).

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$898	35 days	35 days

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All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:White

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COA

Product Introduction

Bioactivity

Description	DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm).
In vitro	BCa cells were pre-incubated with 5 μM DAF-FM DA for 30 minutes to load the probe. The DAF-FM DA probe quantifies nitric oxide (NO) levels in cells. [1]
In vivo	Using zebrafish embryo sections live-stained with DAF-FM DA (5 μM), it was confirmed that the fluorescent signal was primarily localized to areas of ongoing bone formation.[2]
Cell Research	Instructions: I. Solution preparation 1. Preparation of mother solution: Dissolve DAF-FM DA in DMSO to prepare a 10mM mother solution for standby use. 2. Preparation of working solution: Dilute the DAF-FM DA mother solution with PBS/H2O/DMEM to prepare a working solution for standby use. The commonly used concentration is 5-10μM. II. Operation steps 1. Pre-incubate BCa cells with 5 μM DAF-FM DA for 30 minutes to load the probe. 2. Subsequently, incubate the cells with fresh Hank's buffer for 20 minutes to complete deesterification. The DAF-FM DA probe can quantify the level of nitric oxide (NO) in cells. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Diaminofluorescein-FM diacetate

Chemical Properties

Molecular Weight	496.42
Formula	C₂₅H₁₈F₂N₂O₇
Cas No.	254109-22-3
Smiles	O=C1OC2(C3=CC(F)=C(OC(=O)C)C=C3OC4=CC(OC(=O)C)=C(F)C=C42)C5=CC=C(NC)C(N)=C15
Relative Density.	1.56 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Diaminofluorescein-FMDAF-FM DADAFFM DADAF FM DA