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Nitrocefin

Name: Nitrocefin
Brand: TargetMol
SKU: T19709
Price: 66 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19709 Copy Product Info

Purity: 98.22%

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Nitrocefin is a chromogenic substrate and a detection tool for β-lactamase, featuring high sensitivity and rapid color development. Used for β-lactamase activity detection and drug resistance research, with enzyme activity intuitively reflected by the color change from yellow to red.

Cas No. 41906-86-9

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Pack Size	Price	USA Stock	Global Stock
1 mg	$66	In Stock	In Stock
5 mg	$141	In Stock	In Stock
10 mg	$241	In Stock	In Stock
25 mg	$457	In Stock	In Stock
50 mg	$587	In Stock	In Stock
100 mg	$842	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:98.22%

Appearance:Solid

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COA HNMR HPLC

Product Introduction

Nitrocefin AI Summary

Nitrocefin exhibits bioactivity primarily as a beta-lactamase inhibitor, showing significant inhibitory effects across various bacterial beta-lactamase enzymes, including those from Citrobacter freundii, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. The compound displays diverse kinetic parameters, with Km values ranging from 9000.0 nM to 1,300,000 nM and Kcat values from 0.7 /s to 2794.0 /s, depending on the specific enzyme tested. Key data points include a Km value of 32000.0 nM and a Kcat value of 48.0 /s for Citrobacter freundii PER2 beta-lactamase, and a Ki value of 36600.0 nM for recombinant SPM-1. The varied Kcat/Km ratios indicate different levels of enzymatic efficiency, suggesting Nitrocefin's potential as a broad-spectrum beta-lactamase inhibitor. Notably, the compound exhibited an activity level of 224.0% compared to cephalothin and varying effectiveness relative to other antibiotics like cephaloridine. The compound also displayed bioactivity against metallo-beta-lactamases, including VIM-11, VIM-2, and CphA, as well as non-beta-lactamase targets such as human NAPE-PLD with an IC50 of 100000.0 nM. Overall, these findings highlight Nitrocefin's potential for further development in combating antibiotic-resistant bacterial infections..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description

In vitro

Methods: In the Nitro-Carba test, 31 carbapenemase-producing Enterobacterales and 56 non-carbapenemase-producing strains were used as test organisms. After enzyme extraction, the enzyme solutions were added to wells containing or without carbapenem antibiotics, incubated for 5 minutes, then Nitrocefin was added to a final concentration of 1 g/L, and observed at room temperature for 20 minutes.
Results: Carbapenemase-producing strains caused Nitrocefin to change from yellow to red in all wells, while non-carbapenemase-producing strains only showed color change in the antibiotic-free control wells. [1]
Methods: The disk method and liquid method were used to detect Nitrocefin activity in vitro. The test strains included Neisseria gonorrhoeae 8903, WHO O, WHO F, F62, and Escherichia coli DH5α, C600. N. gonorrhoeae was cultured on GC medium at 37°C with 5%–7% CO₂ for 18–24 h; E. coli was cultured on LB medium at 37°C.
Results:: The positive control WHO O showed immediate color change, while N. gonorrhoeae 8903 required 35 min to show positive Results. Transformed E. coli showed Nitrocefin hydrolysis activity at ≥4 h, and transformed F62 at >6 h. [2]

Chemical Properties

Molecular Weight	516.5
Formula	C₂₁H₁₆N₄O₈S₂
Cas No.	41906-86-9
Smiles	OC(=O)C1=C(CS[C@@H]2[C@H](NC(=O)Cc3cccs3)C(=O)N12)\C=C\c1ccc(cc1[N+]([O-])=O)[N+]([O-])=O
Relative Density.	1.67g/cm3

Storage & Solubility Information

Storage

Store at low temperature,Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: ＜1 mg/mL (insoluble)

DMSO: 255 mg/mL (493.71 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9361 mL	9.6805 mL	19.3611 mL	96.8054 mL
5 mM	0.3872 mL	1.9361 mL	3.8722 mL	19.3611 mL
10 mM	0.1936 mL	0.9681 mL	1.9361 mL	9.6805 mL
20 mM	0.0968 mL	0.4840 mL	0.9681 mL	4.8403 mL
50 mM	0.0387 mL	0.1936 mL	0.3872 mL	1.9361 mL
100 mM	0.0194 mL	0.0968 mL	0.1936 mL	0.9681 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.