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Lactate Dehydrogenase, Bovine Heart

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Catalog No. TRP-00466
Alias L-LDH, LDH, LD, LAD, (S)-Lactate: NAD+ oxidoreductase

Lactate Dehydrogenase, Bovine Heart belongs to the oxidoreductase family and catalyzes the reversible conversion between lactic acid and pyruvic acid. It is mainly used in life science research, the diagnostic detection of tissue damage, and also serves as an enzymatic marker in relevant experiments and assays.

Lactate Dehydrogenase, Bovine Heart

Lactate Dehydrogenase, Bovine Heart

Copy Product Info
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Catalog No. TRP-00466Alias L-LDH, LDH, LD, LAD, (S)-Lactate: NAD+ oxidoreductase
Lactate Dehydrogenase, Bovine Heart belongs to the oxidoreductase family and catalyzes the reversible conversion between lactic acid and pyruvic acid. It is mainly used in life science research, the diagnostic detection of tissue damage, and also serves as an enzymatic marker in relevant experiments and assays.
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Color:White
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Product Introduction

Bioactivity
Description
Lactate Dehydrogenase, Bovine Heart belongs to the oxidoreductase family and catalyzes the reversible conversion between lactic acid and pyruvic acid. It is mainly used in life science research, the diagnostic detection of tissue damage, and also serves as an enzymatic marker in relevant experiments and assays.
In vitro
Lactate Dehydrogenase, Bovine Heart is released into the blood when cardiomyocytes are damaged by foot-and-mouth disease, and its elevated activity serves as a non-specific diagnostic marker for tissue injury [1].
This enzyme can undergo complex interactions with lipids on the cell membrane, and this binding process is regulated by the cellular microenvironment (pH, ionic strength, metabolite concentration), thereby affecting its intracellular localization and catalytic activity [2].
SynonymsL-LDH, LDH, LD, LAD, (S)-Lactate: NAD+ oxidoreductase
Chemical Properties
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
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2 Enter the in vivo formulation:
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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