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Sulfo-NHS-Biotin

Name: Sulfo-NHS-Biotin
Brand: TargetMol
SKU: T19952
Price: 50 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19952Cas No. 119616-38-5

Alias Sulfosuccinimidyl biotin, Sulfosuccinimidobiotin, Sulfo NHS Biotin, N-hydroxysulfosuccinimidyl biotin, Biotin-NHS

Sulfo-NHS-Biotin (Biotin-NHS) is a water-soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. It can be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin.

Sulfo-NHS-Biotin

🥰Excellent

Purity: 99.92%

Catalog No. T19952Alias Sulfosuccinimidyl biotin, Sulfosuccinimidobiotin, Sulfo NHS Biotin, N-hydroxysulfosuccinimidyl biotin, Biotin-NHSCas No. 119616-38-5

Pack Size	Price	Availability
5 mg	$50	In Stock
10 mg	$70	In Stock
25 mg	$126	In Stock
50 mg	$174	In Stock
100 mg	$246	In Stock

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Purity:99.92%

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Sulfo-NHS-Biotin (Biotin-NHS) is a water-soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. It can be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin.
Synonyms	Sulfosuccinimidyl biotin, Sulfosuccinimidobiotin, Sulfo NHS Biotin, N-hydroxysulfosuccinimidyl biotin, Biotin-NHS

Chemical Properties

Molecular Weight	443.42
Formula	C₁₄H₁₈N₃NaO₈S₂
Cas No.	119616-38-5
Smiles	C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)[O-])NC(=O)N2.[Na+]
Relative Density.	1.64g/cm3
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (124.04 mM), Sonication is recommended.

H2O: 20 mg/mL (45.1 mM), The compound is unstable in solution. Please use soon.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2552 mL	11.2760 mL	22.5520 mL	112.7599 mL
5 mM	0.4510 mL	2.2552 mL	4.5104 mL	22.5520 mL
10 mM	0.2255 mL	1.1276 mL	2.2552 mL	11.2760 mL
20 mM	0.1128 mL	0.5638 mL	1.1276 mL	5.6380 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0451 mL	0.2255 mL	0.4510 mL	2.2552 mL
100 mM	0.0226 mL	0.1128 mL	0.2255 mL	1.1276 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc