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Sulfo-NHS-Biotin

Name: Sulfo-NHS-Biotin
Brand: TargetMol
SKU: T19952
Price: 50 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19952Cas No. 119616-38-5

Alias Sulfosuccinimidyl biotin, Sulfosuccinimidobiotin, Sulfo NHS Biotin, N-hydroxysulfosuccinimidyl biotin, Biotin-NHS

Sulfo-NHS-Biotin (Biotin-NHS) is a water-soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. It can be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin.

Sulfo-NHS-Biotin

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Purity: 99.92%

Catalog No. T19952Alias Sulfosuccinimidyl biotin, Sulfosuccinimidobiotin, Sulfo NHS Biotin, N-hydroxysulfosuccinimidyl biotin, Biotin-NHSCas No. 119616-38-5

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$50	In Stock	In Stock
10 mg	$70	In Stock	In Stock
25 mg	$126	In Stock	In Stock
50 mg	$174	-	In Stock
100 mg	$246	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.92%

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	Sulfo-NHS-Biotin (Biotin-NHS) is a water-soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. It can be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin.
Synonyms	Sulfosuccinimidyl biotin, Sulfosuccinimidobiotin, Sulfo NHS Biotin, N-hydroxysulfosuccinimidyl biotin, Biotin-NHS

Chemical Properties

Molecular Weight	443.42
Formula	C₁₄H₁₈N₃NaO₈S₂
Cas No.	119616-38-5
Smiles	C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)[O-])NC(=O)N2.[Na+]
Relative Density.	1.64g/cm3

Storage & Solubility Information

Storage

store at low temperature,The compound is unstable in solution. Please use soon | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 237.5 mg/mL (535.61 mM), Sonication is recommended.

H2O: 20 mg/mL (45.1 mM), The compound is unstable in solution. Please use soon.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2552 mL	11.2760 mL	22.5520 mL	112.7599 mL
5 mM	0.4510 mL	2.2552 mL	4.5104 mL	22.5520 mL
10 mM	0.2255 mL	1.1276 mL	2.2552 mL	11.2760 mL
20 mM	0.1128 mL	0.5638 mL	1.1276 mL	5.6380 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0451 mL	0.2255 mL	0.4510 mL	2.2552 mL
100 mM	0.0226 mL	0.1128 mL	0.2255 mL	1.1276 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc