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Biotin-LC-LC-NHS

Name: Biotin-LC-LC-NHS
Brand: TargetMol
SKU: T41026
Price: 35 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T41026Cas No. 89889-52-1

Biotin-LC-LC-NHS is a membrane permeable SMCC cross-linker and an NHS ester-activated biotinylation reagent that biotinylates antibodies or other proteins for detection or purification using streptavidin probes or resins.

Biotin-LC-LC-NHS

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Purity: 99.18%

Catalog No. T41026Cas No. 89889-52-1

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$35	-	In Stock
25 mg	$54	-	In Stock
50 mg	$91	-	In Stock
100 mg	$148	-	In Stock
200 mg	$223	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.18%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	Biotin-LC-LC-NHS is a membrane permeable SMCC cross-linker and an NHS ester-activated biotinylation reagent that biotinylates antibodies or other proteins for detection or purification using streptavidin probes or resins.
In vitro	Biotinylation of the antibody was achieved by reacting Biotin-LC-LC-NHS (1 mg/mL, dissolved in dimethylformamide) with the antibody in the aqueous phase at pH 7.4. The molar ratio of biotin to antibody in the labeling reaction was 10:1, and the final biotin concentration was 70 μM. [1]

Chemical Properties

Molecular Weight	567.7
Formula	C₂₆H₄₁N₅O₇S
Cas No.	89889-52-1
Smiles	C(CCCC(NCCCCCC(NCCCCCC(ON1C(=O)CCC1=O)=O)=O)=O)[C@H]2[C@@]3([C@](CS2)(NC(=O)N3)[H])[H]
Relative Density.	1.29 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (176.15 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (7.05 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7615 mL	8.8075 mL	17.6149 mL	88.0747 mL
5 mM	0.3523 mL	1.7615 mL	3.5230 mL	17.6149 mL
10 mM	0.1761 mL	0.8807 mL	1.7615 mL	8.8075 mL
20 mM	0.0881 mL	0.4404 mL	0.8807 mL	4.4037 mL
50 mM	0.0352 mL	0.1761 mL	0.3523 mL	1.7615 mL
100 mM	0.0176 mL	0.0881 mL	0.1761 mL	0.8807 mL

Calculator

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Dilution Calculator
Reconstitution Calculator
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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Biotin-LC-LC-NHSBiotinLCLCNHSBiotin LC LC NHS