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Streptavidin

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Catalog No. T8781Cas No. 9013-20-1

Streptavidin is a protein tetramer purified from bacteria with a very high affinity for biotin and is commonly used for the purification or detection of various biomolecules. Streptavidin is immunologically active, inhibiting IL-2 synthesis and CD25 expression in T cells.

Streptavidin

Streptavidin

🥰Excellent
Catalog No. T8781Cas No. 9013-20-1
Streptavidin is a protein tetramer purified from bacteria with a very high affinity for biotin and is commonly used for the purification or detection of various biomolecules. Streptavidin is immunologically active, inhibiting IL-2 synthesis and CD25 expression in T cells.
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Product Introduction

Bioactivity
Description
Streptavidin is a protein tetramer purified from bacteria with a very high affinity for biotin and is commonly used for the purification or detection of various biomolecules. Streptavidin is immunologically active, inhibiting IL-2 synthesis and CD25 expression in T cells.
In vitro
Compared to the control group, Streptavidin (2-200 μg/mL) significantly inhibits the proliferation of Lewis T cells towards WF, with an inhibition rate of 76%-83% [1].
In Western blot or ELISA, a concentration of 0.1-1 µg/mL is sufficient.
For immunoprecipitation, a concentration of 1-10 µg/mL can be used.
In vivo
In Lewis rat models, subcutaneous administration of Streptavidin induced a significant immunosuppressive effect, including reduced T cell proliferation responses to third-party antigens[1].
Chemical Properties
Cas No.9013-20-1
Relative Density.1.31g/cm3
Storage & Solubility Information
Storagekeep away from moisture | store at -20°C
Solubility Information
H2O: 80 mg/mL, Sonication is recommended.

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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