Gol-NTR, a highly selective and sensitive Golgi-targetable probe, is activated by Nitroreductase (NTR) to visualize acute lung injury (ALI) and its repair. With a low detection limit of 54.8 ng/mL, Gol-NTR is well-suited for research focused on monitoring and assessing the response of sepsis-induced ALI [1].

Gol-NTR fluorescently labels NTR [1] by preparing a 1.0 mM stock solution in DMSO (1).

In vivo imaging [1] involved the pretreatment of male C57BL/6 mice (6-8 weeks old, weighing 20-22 g) with 300 μL of DMOG (25 mg/mL) followed by an intraperitoneal injection of 300 μL of LPS (10 mg/kg) 24 hours later and was sustained for 6 hours. Post-euthanasia by cervical dislocation, lung tissues were collected. The tissues were then rinsed with PBS and incubated with 50 μM Gol-NTR in PBS for 1 hour. After another PBS wash, fluorescence imaging was conducted using a small animal imaging system at an excitation wavelength of 420 nm and an emission wavelength of 510 nm.