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BP Fluor 405 NHS ester

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Catalog No. T206420Cas No. 2131079-93-9

BP Fluor 405 NHS Ester is a water-soluble blue fluorescent dye commonly utilized in multicolor applications, such as flow cytometry and super-resolution microscopy using STORM. It is optimally excited by the 407 nm spectral line of krypton lasers or 408 nm violet laser diodes. The binding compounds of BP Fluor 405 are pH insensitive from pH 4 to pH 10. NHS esters (or succinimidyl esters) are highly preferred amine-reactive groups for labeling primary amines in proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BP Fluor 405 NHS Ester at high molar ratios without significant self-quenching, resulting in brighter conjugates and more sensitive detection. Additionally, BP Fluor 405 conjugates can be employed for detecting abundant targets.

BP Fluor 405 NHS ester

BP Fluor 405 NHS ester

Copy Product Info
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Catalog No. T206420Cas No. 2131079-93-9
BP Fluor 405 NHS Ester is a water-soluble blue fluorescent dye commonly utilized in multicolor applications, such as flow cytometry and super-resolution microscopy using STORM. It is optimally excited by the 407 nm spectral line of krypton lasers or 408 nm violet laser diodes. The binding compounds of BP Fluor 405 are pH insensitive from pH 4 to pH 10. NHS esters (or succinimidyl esters) are highly preferred amine-reactive groups for labeling primary amines in proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BP Fluor 405 NHS Ester at high molar ratios without significant self-quenching, resulting in brighter conjugates and more sensitive detection. Additionally, BP Fluor 405 conjugates can be employed for detecting abundant targets.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
10 mgInquiry10-14 weeks10-14 weeks
50 mgInquiry10-14 weeks10-14 weeks
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
BP Fluor 405 NHS Ester is a water-soluble blue fluorescent dye commonly utilized in multicolor applications, such as flow cytometry and super-resolution microscopy using STORM. It is optimally excited by the 407 nm spectral line of krypton lasers or 408 nm violet laser diodes. The binding compounds of BP Fluor 405 are pH insensitive from pH 4 to pH 10. NHS esters (or succinimidyl esters) are highly preferred amine-reactive groups for labeling primary amines in proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with BP Fluor 405 NHS Ester at high molar ratios without significant self-quenching, resulting in brighter conjugates and more sensitive detection. Additionally, BP Fluor 405 conjugates can be employed for detecting abundant targets.
Chemical Properties
Molecular Weight726.705
FormulaC28H26N2O15S3
Cas No.2131079-93-9
SmilesO=C(ON1C(=O)CCC1=O)CCCCCNC(=O)COC2=CC(=C3C=CC=4C=5C(=CC=C2C35)C(=CC4S(=O)(=O)O)S(=O)(=O)O)S(=O)(=O)O
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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