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Synonyms:

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 mg | $35 | - | In Stock | |
| 25 mg | $68 | - | In Stock | |
| 50 mg | $106 | - | In Stock | |
| 100 mg | $163 | - | In Stock | |
| 1 mL x 10 mM (in DMSO) | $29 | - | In Stock |
| Description | X-Gluc cyclohexanamine is a dye used to detect β-glucuronidase (GUS) activity and serves as a marker for gene expression, enabling the detection of target gene expression. |
| In vitro | a. Dissolve 20 mg X-Gluc cyclohexanamine in 1 mL dimethylformamide (DMF) to prepare X-Gluc stock solution; b. Add the prepared X-Gluc solution to the plate culture medium, and set the final concentration to 50 μg/mL without sterilization; c. Allow the plate to air dry naturally before inoculating microorganisms; d. Incubate the plate at 35°C and observe the results 16-24 hours after inoculation. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 521.79 |
| Formula | C20H26BrClN2O7 |
| Cas No. | 114162-64-0 |
| Smiles | NC1CCCCC1.O[C@@H]1[C@@H](O)[C@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)O[C@@H]([C@H]1O)C(O)=O |
| Storage | Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 247.5 mg/mL (474.33 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+90% Saline: 10 mg/mL (19.16 mM), Solution. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 µL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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