NCK1 Protein, Human, Recombinant (His)

Name: NCK1 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPJ-00870
Rating: 4.5 (1 reviews)

(Synonyms: SH2/SH3 Adaptor Protein NCK-α, SH2/SH3 Adaptor Protein NCK-Alpha, Nck-1, NCK1, NCK adaptor Protein 1, NCK, Cytoplasmic Protein NCK1) Copy Product Info

Synonyms: SH2/SH3 Adaptor Protein NCK-α, SH2/SH3 Adaptor Protein NCK-Alpha, Nck-1, NCK1, NCK adaptor Protein 1, NCK, Cytoplasmic Protein NCK1

Catalog No. TMPJ-00870 Copy Product Info

Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.

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Pack Size	Price	USA Stock	Global Stock
5 μg	$72	35 days	35 days
10 μg	$116	35 days	35 days
20 μg	$176	35 days	35 days
50 μg	$310	35 days	35 days
100 μg	$532	35 days	35 days
200 μg	$919	35 days	35 days
500 μg	$1,900	35 days	35 days
1 mg	$2,730	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.
Species	Human
Expression System	E. coli
Tag	N-6xHis
Accession Number	P16333
Amino Acid	Met1-Ser377
Construction	Met1-Ser377
Protein Purity	Greater than 80% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 150 mM NaCl, 5% Mannitol, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonyms	SH2/SH3 Adaptor Protein NCK-α, SH2/SH3 Adaptor Protein NCK-Alpha, Nck-1, NCK1, NCK adaptor Protein 1, NCK, Cytoplasmic Protein NCK1
Research Background

Chemical Properties

Molecular Weight

50 KDa (reducing condition)

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

NCK 1

Related Tags: NCK1 Protein, Human, Recombinant (His) chemical structure | NCK1 Protein, Human, Recombinant (His) molecular weight