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5-TAMRA Azide

🥰Excellent
Catalog No. T67840Cas No. 1006592-61-5
Alias TAMRA Azide, isomer 5

5-TAMRA Azide (TAMRA Azide, isomer 5) is the orange-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CuAAC).

5-TAMRA Azide

5-TAMRA Azide

🥰Excellent
Purity: 98.53%
Catalog No. T67840Alias TAMRA Azide, isomer 5Cas No. 1006592-61-5
5-TAMRA Azide (TAMRA Azide, isomer 5) is the orange-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CuAAC).
Pack SizePriceAvailabilityQuantity
1 mg$162In Stock
5 mg$392In Stock
10 mg$563In Stock
25 mg$846In Stock
50 mg$1,120In Stock
100 mg$1,460In Stock
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Purity:98.53%
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Product Introduction

Bioactivity
Description
5-TAMRA Azide (TAMRA Azide, isomer 5) is the orange-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CuAAC).
In vitro
Instructions:
I. Solution preparation
1. Stock solution: Dissolve 5-TAMRA Azide in anhydrous DMSO or DMF to prepare a 1–10 mM stock solution. The specific concentration can be adjusted according to experimental requirements.
Notes:
Powder: Store at -20°C in a dark, dry environment.
Solution: Store at -20°C in a dark environment after aliquoting to avoid repeated freezing and thawing.
II. Click Chemistry labeling
5-TAMRA Azide is a fluorescent labeling molecule commonly used in click chemistry reactions. It can react with molecules containing azide or alkyne groups to generate stable triazole bonds.
1. Reaction conditions:
1) Reaction buffer: Amine-free buffer (such as PBS or Tris-HCl) with a pH of 6.8–8.5 is usually used.
2) Catalyst system: Add Cu(I) catalysts (such as CuSO₄ and ascorbic acid) and stabilizers (such as triethylamine or TBTA) to accelerate the reaction.
3) After mixing, react at room temperature or with slight heating (37°C) for 1–2 hours.
2. Purification:
Remove unbound 5-TAMRA Azide and byproducts by dialysis, gel filtration or HPLC.
Notes
Operation in dark room: 5-TAMRA Azide is light sensitive and should be handled and stored in a dark room or in dark conditions.
Choice of copper catalyst: Use high-purity Cu(I) catalyst to avoid excessive byproducts in the reaction.
Fresh preparation: It is recommended to prepare it before use to ensure reaction efficiency.
AliasTAMRA Azide, isomer 5
Chemical Properties
Molecular Weight554.64
FormulaC31H34N6O4
Cas No.1006592-61-5
Smiles[N-]=[N+]=NCCCCCCNC(=O)C=1C=CC(=C(C1)C(=O)[O-])C=2C=3C=CC(=CC3[O+]=C4C=C(C=CC42)N(C)C)N(C)C
Relative Density.no data available
Storage & Solubility Information
Storagestore at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
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2 Enter the in vivo formulation:
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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