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5-TAMRA Azide (TAMRA Azide, isomer 5) is the orange-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CuAAC).
Pack Size | Price | Availability | Quantity |
---|---|---|---|
1 mg | $162 | In Stock | |
5 mg | $392 | In Stock | |
10 mg | $563 | In Stock | |
25 mg | $846 | In Stock | |
50 mg | $1,120 | In Stock | |
100 mg | $1,460 | In Stock |
Description | 5-TAMRA Azide (TAMRA Azide, isomer 5) is the orange-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CuAAC). |
In vitro | Instructions: I. Solution preparation 1. Stock solution: Dissolve 5-TAMRA Azide in anhydrous DMSO or DMF to prepare a 1–10 mM stock solution. The specific concentration can be adjusted according to experimental requirements. Notes: Powder: Store at -20°C in a dark, dry environment. Solution: Store at -20°C in a dark environment after aliquoting to avoid repeated freezing and thawing. II. Click Chemistry labeling 5-TAMRA Azide is a fluorescent labeling molecule commonly used in click chemistry reactions. It can react with molecules containing azide or alkyne groups to generate stable triazole bonds. 1. Reaction conditions: 1) Reaction buffer: Amine-free buffer (such as PBS or Tris-HCl) with a pH of 6.8–8.5 is usually used. 2) Catalyst system: Add Cu(I) catalysts (such as CuSO₄ and ascorbic acid) and stabilizers (such as triethylamine or TBTA) to accelerate the reaction. 3) After mixing, react at room temperature or with slight heating (37°C) for 1–2 hours. 2. Purification: Remove unbound 5-TAMRA Azide and byproducts by dialysis, gel filtration or HPLC. Notes Operation in dark room: 5-TAMRA Azide is light sensitive and should be handled and stored in a dark room or in dark conditions. Choice of copper catalyst: Use high-purity Cu(I) catalyst to avoid excessive byproducts in the reaction. Fresh preparation: It is recommended to prepare it before use to ensure reaction efficiency. |
Alias | TAMRA Azide, isomer 5 |
Molecular Weight | 554.64 |
Formula | C31H34N6O4 |
Cas No. | 1006592-61-5 |
Smiles | [N-]=[N+]=NCCCCCCNC(=O)C=1C=CC(=C(C1)C(=O)[O-])C=2C=3C=CC(=CC3[O+]=C4C=C(C=CC42)N(C)C)N(C)C |
Relative Density. | no data available |
Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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