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4,5-Diaminofluorescein (Synonyms: DAF-2)

Name: 4,5-Diaminofluorescein
Brand: TargetMol
SKU: T85447
Price: 147 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T85447 Copy Product Info

Purity: 99.88%

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4,5-Diaminofluorescein (DAF-2) is a highly sensitive fluorescent probe for selective Nitric Oxide (NO) detection. It reacts with NO to generate a triazole derivative for real-time monitoring.

4,5-Diaminofluorescein

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Catalog No. T85447

Synonyms DAF-2

4,5-Diaminofluorescein (DAF-2) is a highly sensitive fluorescent probe for selective Nitric Oxide (NO) detection. It reacts with NO to generate a triazole derivative for real-time monitoring.

Cas No. 205391-01-1

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Pack Size	Price	USA Stock	Global Stock
500 μg	$147	-	In Stock
1 mg	$228	-	In Stock
5 mg	$689	-	In Stock
10 mg	$973	-	In Stock
25 mg	$1,430	-	In Stock
50 mg	$1,930	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.88%

Appearance:Solid

Color:Orange

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	4,5-Diaminofluorescein (DAF-2) is a highly sensitive fluorescent probe for selective Nitric Oxide (NO) detection. It reacts with NO to generate a triazole derivative for real-time monitoring.
In vitro	4,5-Diaminofluorescein (0.1 uM) reacts with NO to form fluorescent DAF-2T, allowing real-time NO monitoring [1][2].
Synonyms	DAF-2

Chemical Properties

Molecular Weight	362.34
Formula	C₂₀H₁₄N₂O₅
Cas No.	205391-01-1
Smiles	O=C1OC2(C3=CC=C(O)C=C3OC4=CC(O)=CC=C42)C5=CC(N)=C(N)C=C15

Storage & Solubility Information

Storage

Keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 20 mg/mL (55.2 mM), Sonication is recommmended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7598 mL	13.7992 mL	27.5984 mL	137.9919 mL
5 mM	0.5520 mL	2.7598 mL	5.5197 mL	27.5984 mL
10 mM	0.2760 mL	1.3799 mL	2.7598 mL	13.7992 mL
20 mM	0.1380 mL	0.6900 mL	1.3799 mL	6.8996 mL
50 mM	0.0552 mL	0.2760 mL	0.5520 mL	2.7598 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc