Reactive Oxygen Species Assay Kit

The Targeted Mol Reactive Oxygen Species (ROS) Detection Kit is a kit that uses DCFH-DA as a fluorescent probe to detect intracellular ROS levels. ROS is a class of highly reactive molecules or ions derived from oxygen, including hydrogen peroxide (H2O2), superoxide anions, and hydroxyl radicals. At physiological concentrations, ROS acts as an important signaling molecule and participates in various important physiological processes such as immune defense in the body; When ROS is produced excessively and exceeds the body's antioxidant system's clearance capacity, it can trigger oxidative stress, causing DNA and protein damage, and ultimately leading to disease.
DCFH-DA (2 ', 7' - Dichlorodihydrofluorescein diacetate) is a lipophilic fluorescent probe that contains two acetate groups in its molecular structure, allowing it to freely penetrate the cell membrane and enter the cell. In cells, DCFH-DA is first hydrolyzed by non-specific esterases to remove acetate groups and generate non fluorescent 2 ', 7' - dichlorodihydrofluorescein (DCFH). DCFH cannot freely pass through the cell membrane and can therefore remain inside the cell. When ROS is present inside the cell, DCFH is oxidized to 2 ', 7' - dichlorofluorescein (DCF). DCF cannot penetrate the cell membrane and emits bright green fluorescence upon excitation (excitation wavelength of 488 nm, emission wavelength of 525 nm). The fluorescence intensity is proportional to the level of reactive oxygen species in the cell. Therefore, by detecting the fluorescence intensity of DCF, the level of intracellular reactive oxygen species can be quantitatively analyzed.
This kit is suitable for various detection instruments such as fluorescence microscope, laser confocal microscope, fluorescence spectrophotometer, and flow cytometer. If this reagent kit is used for a 6-well plate with a detection volume of 1 mL per well, it can detect 100 times; If used for a 96 well plate with a detection volume of 100 µ L per well, it can be tested 1000 times.