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6-FITC (Synonyms: 6-Fluorescein Isothiocyanate)

Catalog No. T14058 Copy Product Info

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6-FITC is a commonly used green fluorescent labeling dye and a fluorescein isothiocyanate derivative with excitation and emission wavelengths of 495 nm and 520 nm, respectively. 6-FITC can covalently bind to amino groups in proteins, antibodies, or peptides, making it suitable for a variety of applications, including flow cytometry, fluorescence microscopy, and tissue section staining. Since FITC itself is not cell-permeable, it is typically used to label surface antigens on live cells; however, it can also be used for intracellular protein detection after cell fixation or permeabilization.

6-FITC

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Catalog No. T14058

Synonyms 6-Fluorescein Isothiocyanate

Cas No. 3326-31-6

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Pack Size	Price	USA Stock	Global Stock
5 mg	$30	-	In Stock
10 mg	$42	-	In Stock
25 mg	$74	-	In Stock
1 mL x 10 mM (in DMSO)	$162	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Appearance:Solid

Color:Orange

Resource Download

COA

Product Introduction

Bioactivity

Description

Synonyms

6-Fluorescein Isothiocyanate

Chemical Properties

Molecular Weight	389.38
Formula	C₂₁H₁₁NO₅S
Cas No.	3326-31-6
Smiles	O=C1OC2(C3=CC=C(O)C=C3OC4=CC(O)=CC=C42)C=5C=CC=C(N=C=S)C15
Relative Density.	1.54 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (205.45 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5682 mL	12.8409 mL	25.6819 mL	128.4093 mL
5 mM	0.5136 mL	2.5682 mL	5.1364 mL	25.6819 mL
10 mM	0.2568 mL	1.2841 mL	2.5682 mL	12.8409 mL
20 mM	0.1284 mL	0.6420 mL	1.2841 mL	6.4205 mL
50 mM	0.0514 mL	0.2568 mL	0.5136 mL	2.5682 mL
100 mM	0.0257 mL	0.1284 mL	0.2568 mL	1.2841 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

6FITC6 FITC