cell-surface protein

Sulfo-NHS-SS-Biotin sodium (Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate) is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein.

AF647 C2 maleimide is a water-soluble fluorescent dye that remains stable under a range of physiological conditions. It facilitates the attachment of fluorescent groups to proteins by reacting with thiol groups on target proteins. This labeling method offers high sensitivity and specificity, enabling the detection of proteins at very low concentrations, making it valuable for studying protein expression and localization. Additionally, it can bind to antibodies for labeling cell surface antigens or specific proteins within tissues. [Ex Em(nm)=651 671].

PM-1, a Thioflavin-T (ThT) derivative, is a small, highly specific fluorescent dye for plasma membrane (PM) imaging in living and fixed cells. It integrates directly into the cell membrane and retains its presence on the PM for about 15 hours. PM-1 can be combined with protein labeling probes to investigate ectodomain shedding and endocytosis of cell surface proteins [1].