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Sulfo-NHS-SS-Biotin sodium

Name: Sulfo-NHS-SS-Biotin sodium
Brand: TargetMol
SKU: T19061
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19061Cas No. 325143-98-4

Alias Sulfo-NThiol-SS-biotin

Sulfo-NHS-SS-Biotin sodium (Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate) is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein.

Sulfo-NHS-SS-Biotin sodium

🥰Excellent

Purity: 99.57%

Catalog No. T19061Alias Sulfo-NThiol-SS-biotinCas No. 325143-98-4

Pack Size	Price	Availability	Quantity
5 mg	$33	In Stock
10 mg	$44	In Stock

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Purity:99.57%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	Sulfo-NHS-SS-Biotin sodium (Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate) is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein.
Targets&IC50	Cell-surface protein:
In vitro	Sulfo-NHS-SS-biotin is a negatively charged reagent that does not permeate cell membranes and reacts with primary amines (NH2), such as lysine side-chains or amino-termini of polypeptides[1]. Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied to cells in monolayer culture, washed by ice-cold PBS. The biotinylation reaction is terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail[2].
Cell Research	Methods CHO cells were treated with Sulfo-NHS-SS-Biotin sodium (0.78 mg/mL, 37°C, 1h) and washed with PBS to remove residual dye. Proteins in the cells were then quantified using LC-MS/MS.
Synonyms	Sulfo-NThiol-SS-biotin

Chemical Properties

Molecular Weight	606.69
Formula	C₁₉H₂₇N₄NaO₉S₄
Cas No.	325143-98-4
Smiles	[Na+].[O-]S(=O)(=O)C1CC(=O)N(OC(=O)CCSSCCNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23)C1=O
Relative Density.	no data available
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 180 mg/mL (296.7 mM), Sonication is recommended.

DMSO: 250 mg/mL (412.07 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.6483 mL	8.2414 mL	16.4829 mL	82.4144 mL
5 mM	0.3297 mL	1.6483 mL	3.2966 mL	16.4829 mL
10 mM	0.1648 mL	0.8241 mL	1.6483 mL	8.2414 mL
20 mM	0.0824 mL	0.4121 mL	0.8241 mL	4.1207 mL
50 mM	0.0330 mL	0.1648 mL	0.3297 mL	1.6483 mL
100 mM	0.0165 mL	0.0824 mL	0.1648 mL	0.8241 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Sulfo-NHS-SS-Biotin sodiumcell-surface protein