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Anti-HER4/ERBB4 Antibody (8W756)
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Catalog No. TMAC-01813
Alias Tyrosine kinase-type cell surface receptor HER4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, p180erbB4
Anti-HER4/ERBB4 Antibody (8W756) is a Mouse antibody targeting HER4/ERBB4. Anti-HER4/ERBB4 Antibody (8W756) can be used in FCM,IF,IHC-P,IP,WB.
Anti-HER4/ERBB4 Antibody (8W756)
Anti-HER4/ERBB4 Antibody (8W756)
😃Good
Catalog No. TMAC-01813Alias Tyrosine kinase-type cell surface receptor HER4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, p180erbB4
Anti-HER4/ERBB4 Antibody (8W756) is a Mouse antibody targeting HER4/ERBB4. Anti-HER4/ERBB4 Antibody (8W756) can be used in FCM,IF,IHC-P,IP,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $428 | 7-10 days |
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Chemical Properties
| Description | Anti-HER4/ERBB4 Antibody (8W756) is a Mouse antibody targeting HER4/ERBB4. Anti-HER4/ERBB4 Antibody (8W756) can be used in FCM,IF,IHC-P,IP,WB. |
| Synonyms | Tyrosine kinase-type cell surface receptor HER4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, p180erbB4 |
| Clone | 8W756 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of ErbB-4 expression in control (A) and ErbB-4 transfected (B) NIH/3T3 whole cell lysates. 2. Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and ErbB-4 transfected (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal mouse IgG2a. |
| Application | |
| Recommended Dose | WB: 1:100-1000; IHC-P: 1:50-500; IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate); FCM: 1 μg per 1 x 106 cells |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. |
| Conjucates | Unconjugated |
| Immunogen | Amino acids 1280-1308 at C-terminus of ErbB-4 of human origin |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 180 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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