Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 418.00 | |
500 μg | 5 days | $ 1,670.00 | |
1 mg | 5 days | $ 2,800.00 |
Description | Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). |
Species | Human |
Expression System | HEK293 |
Tag | N-hFc |
Accession Number | AAB59397.1 |
Synonyms | Apolipoprotein E, Apo-E, apolipo E, APOE4, APOE |
Construction | Recombinant Human APOE4/Apolipoprotein E Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Lys19-His317.[Accession |AAB59397.1] |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS, 2mM DTT, 5mM CHAPS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). |
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Please read the User Guide of Recombinant Proteins for more specific information.
Apolipoprotein E/APOE4 Protein, Human, Recombinant (hFc) Apolipoprotein E APOE 4 Apo-E APOE-4 apolipo E APOE4 APOE recombinant recombinant-proteins proteins protein