CD3 epsilon/CD3e Protein, Cynomolgus, Recombinant (aa 22-117, hFc)

Catalog No. TMPJ-01259

Synonyms: CD3 epsilon, T-cell antigen receptor complex, epsilon subunit of T3, CD3e antigen, ε polypeptide (TiT3 complex), T-cell surface antigen T3/Leu-4 epsilon chain, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, ε subunit of T3, CD3e antigen, FLJ18683, CD3e, CD3e molecule, epsilon (CD3-TCR complex), CD3 ε, CD3e molecule, ε (CD3-TCR complex), T3E, CD3-epsilon, T-cell surface antigen T3/Leu-4 ε chain, CD3-ε

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

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CD3 epsilon/CD3e Protein, Cynomolgus, Recombinant (aa 22-117, hFc)

Pack Size	Availability	Price/USD
10 μg	5 days	$ 129.00
50 μg	5 days	$ 390.00
500 μg	5 days	$ 1,900.00
1 mg	5 days	$ 2,730.00

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Biological Description

Technical Params

Product Properties

Description

Species	Cynomolgus
Expression System	Human Cells
Tag	C-Fc
Accession Number	Q95LI5
Synonyms	CD3 epsilon, T-cell antigen receptor complex, epsilon subunit of T3, CD3e antigen, ε polypeptide (TiT3 complex), T-cell surface antigen T3/Leu-4 epsilon chain, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, ε subunit of T3, CD3e antigen, FLJ18683, CD3e, CD3e molecule, epsilon (CD3-TCR complex), CD3 ε, CD3e molecule, ε (CD3-TCR complex), T3E, CD3-epsilon, T-cell surface antigen T3/Leu-4 ε chain, CD3-ε
Amino Acid	Gln22-Asp117
Construction	Recombinant Cynomolgus T-cell Surface Glycoprotein CD3 Epsilon Chain is produced by our Mammalian expression system and the target gene encoding Gln22-Asp117 is expressed with a human IgG1 Fc tag at the C-terminus.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	38-55 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 100 mM Glycine, pH 7.5.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

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Keywords

CD3 epsilon/CD3e Protein, Cynomolgus, Recombinant (aa 22-117, hFc) CD3 epsilon CD3e molecule, epsilon T-cell antigen receptor complex, epsilon subunit of T3 CD3e antigen, ε polypeptide (TiT3 complex) T-cell surface antigen T3/Leu-4 epsilon chain CD3e antigen, epsilon polypeptide CD3-TCR complex CD3e antigen, epsilon polypeptide (TiT3 complex) T-cell antigen receptor complex, ε subunit of T3 CD3e antigen FLJ18683 TiT3 complex CD3e CD3e molecule, epsilon (CD3-TCR complex) CD3 ε CD3e molecule, ε (CD3-TCR complex) T3E FLJ 18683 FLJ-18683 CD3-epsilon T-cell surface antigen T3/Leu-4 ε chain CD3-ε recombinant recombinant-proteins proteins protein