SHP-2 Protein, Mouse, Recombinant (His)

Name: SHP-2 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-03125
Rating: 4.5 (1 reviews)

(Synonyms: Syp, SH-PTP3, SH-PTP2, SHP-2, Shp2, SAP-2, PTP2C, PTP1D, protein tyrosine phosphatase, non-receptor type 11, AW536184, 2700084A17Rik) Copy Product Info

Synonyms: Syp, SH-PTP3, SH-PTP2, SHP-2, Shp2, SAP-2, PTP2C, PTP1D, protein tyrosine phosphatase, non-receptor type 11, AW536184, 2700084A17Rik

Catalog No. TMPY-03125 Copy Product Info

SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulation of its RhoA binding activity.

Customer service consultation

Pack Size	Price	USA Stock	Global Stock
5 µg	$59	7-10 days	7-10 days
10 µg	$95	7-10 days	7-10 days
20 µg	$155	7-10 days	7-10 days
50 µg	$296	7-10 days	7-10 days
100 µg	$497	7-10 days	7-10 days
200 µg	$987	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Resource Download

User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site . The specific activity is > 1 pmoles/min/μg.
Description	SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulation of its RhoA binding activity.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P35235-2
Construction	A DNA sequence encoding the mouse PTPN11 (P35235-2) (Met1-Arg593) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Met
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 0.1M NaCl, 10% Glycerol, 1 mM TCEP, pH 7.8. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	Syp, SH-PTP3, SH-PTP2, SHP-2, Shp2, SAP-2, PTP2C, PTP1D, protein tyrosine phosphatase, non-receptor type 11, AW536184, 2700084A17Rik
Research Background	SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulation of its RhoA binding activity.

Chemical Properties

Molecular Weight

69.5 kDa (predicted); 65 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.