B2M/beta 2-Microglobulin Protein, Mouse, Recombinant (hFc)

The genetic and functional analysis of β2-microglobulin (B2M), a component of the HLA class-I complex.Acquired homozygous loss of B2M that caused lack of cell-surface HLA Class I expression in the tumor and a matched patient-derived xenograft (PDX). Downregulation of B2M was also found in two additional PDXs established from ICI-resistant tumors.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 324.00
500 μg	5 days	$ 1,300.00
1 mg	5 days	$ 2,170.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 324.00

500 μg

5 days

$ 1,300.00

1 mg

5 days

$ 2,170.00

Description

Species	Mouse
Expression System	HEK293
Tag	C-hFc
Accession Number	P01887
Synonyms	B2M/β 2-Microglobulin, Beta-2-microglobulin, B2M, β-2-microglobulin
Construction	Recombinant Mouse B2M/beta 2-Microglobulin Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ile21-Met119.[Accession \|P01887]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The genetic and functional analysis of β2-microglobulin (B2M), a component of the HLA class-I complex.Acquired homozygous loss of B2M that caused lack of cell-surface HLA Class I expression in the tumor and a matched patient-derived xenograft (PDX). Downregulation of B2M was also found in two additional PDXs established from ICI-resistant tumors.