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Anti-CD99 Antibody (5Q457) (Synonyms: T-cell surface glycoprotein E2, Protein MIC2, E2 antigen, CD99 antigen|...)
Catalog No. TMAC-00746 Copy Product Info
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Anti-CD99 Antibody (5Q457) is a Rabbit antibody targeting CD99. Anti-CD99 Antibody (5Q457) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-CD99 Antibody (5Q457)
Copy Product Info🥰Excellent
Catalog No. TMAC-00746
Synonyms T-cell surface glycoprotein E2, Protein MIC2, E2 antigen, CD99 antigen, 120000000
Anti-CD99 Antibody (5Q457) is a Rabbit antibody targeting CD99. Anti-CD99 Antibody (5Q457) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-CD99 Antibody
(5Q457)
(5Q457)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Bioactivity
Related Conjugates and Formulations
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Chemical Properties
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| Description | Anti-CD99 Antibody (5Q457) is a Rabbit antibody targeting CD99. Anti-CD99 Antibody (5Q457) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | T-cell surface glycoprotein E2, Protein MIC2, E2 antigen, CD99 antigen, 120000000 |
| Ig Type | IgG |
| Clone | 5Q457 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of CD99 on THP-1 cells lysates using anti-CD99 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD99 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD99 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD99 antibody. Counter stained with hematoxylin. 5. ICC staining CD99 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CD99 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining CD99 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Jurkat cells with CD99 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.         |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | MIC2, also designated CD99, is a T cell surface protein that is involved in the aggregation of lymphocytes. Two forms of MIC2, which are differentially expressed, are produced by alternative splicing. The major form induces cellular adhesion, whereas the truncated form inhibits the adhesion process. MIC2 regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes. Overexpression of the truncated form results in downregulated expression of LFA-1. Cells with downregulated MIC2 exhibit a Hodgkin's and Reed-Sternberg (H-RS) phenotype, indicating that MIC2 plays an important role in regulating cell function and morphology. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 25 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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