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Anti-ADGRE1 Polyclonal Antibody 2

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Catalog No. TMAB-00061

Anti-ADGRE1 Polyclonal Antibody 2 is a Rabbit antibody targeting ADGRE1. Anti-ADGRE1 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.

Anti-ADGRE1 Polyclonal Antibody 2

Anti-ADGRE1 Polyclonal Antibody 2

😃Good
Catalog No. TMAB-00061
Anti-ADGRE1 Polyclonal Antibody 2 is a Rabbit antibody targeting ADGRE1. Anti-ADGRE1 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,IF

Reactivity: Mouse
Ig Type
IgG
Reactivity
Mouse
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADGRE1 Polyclonal Antibody, Unconjugated (TMAB-00061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADGRE1 Polyclonal Antibody, Unconjugated (TMAB-00061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADGRE1 Polyclonal Antibody, Unconjugated (TMAB-00061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADGRE1 Polyclonal Antibody, Unconjugated (TMAB-00061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1–3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1–3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ADGRE1
Antigen Species
Human
Gene ID
2015
Uniprot ID
Biology Area
Macrophage / Inflamm.,Other Cell Types
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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