SIRP alpha Protein, Cynomolgus, Recombinant (aa 31-369, His)

Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation

Pack Size	Availability	Price/USD
10 μg	5 days	$ 75.00
50 μg	5 days	$ 231.00
500 μg	5 days	$ 1,840.00
1 mg	5 days	$ 2,770.00

Pack Size

Availability

Price/USD

Quantity

10 μg

5 days

$ 75.00

50 μg

5 days

$ 231.00

500 μg

5 days

$ 1,840.00

1 mg

5 days

$ 2,770.00

Description

Species	Cynomolgus
Expression System	Human Cells
Tag	C-6His
Accession Number	I7G9Z7
Synonyms	Inhibitory Feceptor SHPS-1, MyD-1 Antig, Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs, Macrophage Fusion Receptor, Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1, Bit, SHP Substrate 1, SHPS-1, CD172 Antigen-Like Family Member A
Amino Acid	Glu31Arg369
Construction	Recombinant Cynomolgus Signal-Regulatory Protein Alpha 1 is produced by our Mammalian expression system and the target gene encoding Glu31Arg369 is expressed with a 6His tag at the C-terminus.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	50-75 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation

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SIRP alpha Protein, Cynomolgus, Recombinant (aa 31-369, His)

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