trypsin

Trypsin is a serine protease that can be isolated from fish to hydrolyze lysine or arginine carboxy-side proteins. With the anti-inflammatory activity, Trypsin could induce the cell membrane fusion of PDCoV-infected cells through the interaction between the S glycoprotein of PDCoV and pAPN, activate PAR2 and PAR4, and promote cell proliferation and differentiation. Trypsin can be used to promote wound healing and study neurogenic inflammation.

Aprotinin (Traskolan) is a broad-spectrum serine protease (BPTI) inhibitor that inhibits the activity of a number of different esterases and proteases. Aprotinin is an antifibrinolytic agent used to minimize hemorrhage during complex surgical procedures.

BCX 1470 inhibits the esterolytic activity of factor D and C1s (IC50: 96 nM and 1.6 nM), demonstrating 3.4- and 200-fold greater efficacy compared to trypsin, respectively.

Pirodomast is a thromboxane A (TXA2) synthase inhibitor. Pirodomast inhibits leukotriene (LT) D4, C4, E4 formation and thromboxane B2 (TXB2) activity, but is weakly or ineffectively antagonistic to guinea pig bronchospasm induced by histamine, methacholine, serotonin, LTC4, or platelet-activating factor. Pirodomast is a potential antiallergic compound. Pirodomast had only weak relaxant activity on guinea pig trachea in vitro. Pirodomast is a potential antiallergic compound that inhibits the protein hydrolyzing activity of trypsin in vitro, prevents antigen-induced immediate and late asthmatic responses in allergic sheep in vivo, and inhibits antigen-induced airway hyperresponsiveness to histamine and carbachol in allergic sheep.

4-Aminobenzamidine dihydrochloride (p-Aminobenzamidine dihydrochloride) is a strong trypsin inhibitor and a relatively weak inhibitor of urokinase type plasminogen activator (uPA) (K i =82 μM). 4-Aminobenzamidine can inhibit growth of a human prostate tumor in SCID mice [1] [2].

Benzamidine hydrochloride (Benzamidine HCl) is a reversible inhibitor of serine proteases, including trypsin, plasmin, and thrombin (Ki of 35, 350, and 220 μM, respectively).

BCX 1470 methanesulfonate inhibits the esterolytic activity of factor D and C1s (IC50: 96 nM and 1.6 nM), exhibiting 3.4- and 200-fold greater efficacy than trypsin, respectively.

Demethylwedelolactone is a natural product isolated from Eclipta alba, has trypsin inhibitory effect an IC50 of 3.0 μM. it exerts anti-invasive growth effect on breast cancer cells.

Alpha-Amyrin (alpha-Amyrine) is an inhibitor of trypsin and chymotrypsin with antineoplastic effects. alpha-Amyrin can be used in studies about acting as a hepatomodulatory.

VR23 is a potent proteasome inhibitor. Data shows IC50 =1 nM for trypsin-like proteasomes, IC50=50-100 nM for chymotrypsin-like proteasomes, and IC50=3 μM for caspase-like proteasomes.

Diminazene is a trypanocidal agent.

Marizomib is a novel irreversible brain-permeable proteasome inhibitor that inhibits CT-L (β5), CT-T-laspase-like (C-L, β1), and trypsin-like (T-L, β2) 20S proteasomes with IC50s of 3.5, 28, and 430 nM.[3]

N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride (N-alpha-Tosyl-L-lysine_chloromethyl_ketone_hydrochloride) is an inhibitor of trypsin-like protease and exhibits an inhibitory effect on IFN-γ activities.

TCH-165 is a small molecule modulator of proteasome assembly, resulting in an increase in 20S levels. The increase in the level of free 20S corresponds to enhanced proteolysis of IDPs.

2,4-Dioxaspiro(5.5)undec-8-ene, 3-(2-furanyl)- (Ulinastatin) ,as an urinary trypsin inhibitor (UTI), is a glycoprotein that is isolated from healthy human urine or synthetically produced.

Urinary Trypsin Inhibitor Fragment, a proteolytically derived segment of urinary trypsin inhibitor, demonstrates the capability to inhibit tumor cell invasion through limited proteolysis [1].

Trypsin/Lys-C complex protease (MS grade) combines two recombinant proteases, Trypsin and Lys-C, to efficiently hydrolyze peptide bonds. Trypsin specifically cleaves at the C-terminal of arginine (R) and lysine (K), while Lys-C targets the C-terminal of lysine (K). This combination overcomes issues of missed cleavages due to Trypsin's slower digestion at lysine and arginine, PTM alterations on lysine, or hydrophobic C-termini like proline. It is suitable for digesting complex protein samples that are difficult to process enzymatically. This protease is used for protein characterization, single-cell omics, and large-cohort proteomics studies.

Trypsin Inhibitor, soybean is a trypsin inhibitor extracted from leguminous plants.

Trypsin-Chymotrypsin 6:1 is a biocatalyst and a key enzyme in new biocatalyst technology. Enzyme engineering focuses on improving reaction kinetics, substrate selectivity, and activity under extreme conditions (e.g., low or high pH). Introducing stimulus-responsive modifications to these enzymes also enables dynamic activity control.

Trypsin-Chymotrypsin 1:1 is a biocatalyst and a pivotal enzyme in new biocatalytic technology. Enzyme engineering focuses on improving enzyme reaction kinetics, substrate selectivity, and activity under extreme conditions (e.g., low or high pH). By introducing stimulus responsiveness into these enzyme modifications, dynamic control of activity is also achievable.

Trypsin-Chymotrypsin 1:250 is a biocatalyst, and a crucial enzyme in new biocatalyst technology. Enzyme engineering focuses on improving enzyme reaction kinetics, substrate selectivity, and activity under harsh conditions (e.g., low or high pH). By incorporating stimulus-responsive modifications into these enzymes, dynamic control of activity has also become feasible.

Recombinant Trypsin is a serine protease that hydrolyzes proteins at the carboxyl side of lysine or arginine.

TPCK-treated Trypsin is a form of trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone (TPCK). This compound minimizes autolysis and nonspecific proteolysis during experiments and exhibits stability during storage and handling. TPCK-treated Trypsin is suitable for use in proteomics research.

Chymotrypsin (Chymotrypsin A) is a serine protease synthesized by the pancreas, which cleaves protein chains on the carboxyl side of aromatic amino acids.