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TPCK-treated Trypsin
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Catalog No. TRP-00624
TPCK-treated Trypsin is a form of trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone (TPCK). This compound minimizes autolysis and nonspecific proteolysis during experiments and exhibits stability during storage and handling. TPCK-treated Trypsin is suitable for use in proteomics research.
TPCK-treated Trypsin
TPCK-treated Trypsin
Copy Product Info😃Good
Catalog No. TRP-00624
TPCK-treated Trypsin is a form of trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone (TPCK). This compound minimizes autolysis and nonspecific proteolysis during experiments and exhibits stability during storage and handling. TPCK-treated Trypsin is suitable for use in proteomics research.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | TPCK-treated Trypsin is a form of trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone (TPCK). This compound minimizes autolysis and nonspecific proteolysis during experiments and exhibits stability during storage and handling. TPCK-treated Trypsin is suitable for use in proteomics research. |
| In vitro | Trypsin is treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit its proteolytic activity. The theoretical molecular weight is 23.3 kDa, and the optimal pH range is 7.5 to 8.5. It can be used for protein analysis, such as protein mass spectrometry, sequencing, and peptide profiling analysis. Instructions for use (for reference only): The product can be directly used for protein digestion. The recommended digestion ratio is 1:50-1:100 (w/w); the digestion should be carried out overnight at 37℃. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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