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TPCK

Name: TPCK
Brand: TargetMol
SKU: T8350
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T8350Cas No. 402-71-1

TPCK is an irreversible inhibitor of chymotrypsin-like proteases that affect cell proliferation, apoptosis, and tumorigenesis

TPCK

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Purity: 97.33%

Catalog No. T8350Cas No. 402-71-1

TPCK is an irreversible inhibitor of chymotrypsin-like proteases that affect cell proliferation, apoptosis, and tumorigenesis

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$39	In Stock	In Stock
50 mg	$55	In Stock	In Stock
100 mg	$80	In Stock	In Stock
200 mg	$115	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:97.33%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

TPCK AI Summary

TPCK exhibits a broad spectrum of bioactivities, displaying inhibitory action against several proteases, including alpha-chymotrypsin, human serine protease chymase, human leukocyte cathepsin G, porcine pancreatic elastase, and porcine pancreatic trypsin. It shows a higher inhibitory activity against alpha-chymotrypsin with an inhibitory constant (I) of 0.000057 M and an observed rate constant (K_obs) of 7.7 M^-1 s^-1. This compound also demonstrates antimicrobial activity against Plasmodium falciparum 3D7, showing 21.0% activity at 100 μM after 2 days. Additionally, TPCK displays significant inhibitory effects on TNF-alpha-induced NF-kappaB activation in human HEK293 cells with IC50 values ranging from 3760.0 nM to 5090.0 nM, implying potential anti-inflammatory properties by targeting the NF-kappaB signaling pathway. It also inhibits LPS-induced COX-2 mRNA expression in mouse RAW264.7 cells with an inhibition rate of 59.5% at 20 μM. Furthermore, the compound exhibits potent inhibition of the SARS-CoV-2 3CL-Pro protease with an IC50 value of 1370.0 nM and shows antiviral activity against SARS-CoV-2 by inhibiting virus-induced cytotoxicity in VERO-6 cells. TPCK has also demonstrated antitumor activity in mice and displays cytotoxicity against human HEK293 and HepG2 cells with IC50 values of 3800.0 nM and 7900.0 nM, respectively. Additionally, it inhibits CDK8 in TNF-alpha-induced HEK293 cells with IC50 values of 13000.0 nM and 12000.0 nM. These diverse bioactivities suggest that TPCK holds potential for therapeutic applications in inflammation, cancer, and infectious diseases..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description

TPCK is an irreversible inhibitor of chymotrypsin-like proteases that affect cell proliferation, apoptosis, and tumorigenesis

Chemical Properties

Molecular Weight	351.85
Formula	C₁₇H₁₈ClNO₃S
Cas No.	402-71-1
Smiles	Cc1ccc(cc1)S(=O)(=O)N[C@@H](Cc1ccccc1)C(=O)CCl
Relative Density.	1.1350 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (710.53 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (5.68 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8421 mL	14.2106 mL	28.4212 mL	142.1060 mL
5 mM	0.5684 mL	2.8421 mL	5.6842 mL	28.4212 mL
10 mM	0.2842 mL	1.4211 mL	2.8421 mL	14.2106 mL
20 mM	0.1421 mL	0.7105 mL	1.4211 mL	7.1053 mL
50 mM	0.0568 mL	0.2842 mL	0.5684 mL	2.8421 mL
100 mM	0.0284 mL	0.1421 mL	0.2842 mL	1.4211 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc