Tryptases are serine proteases with trypsin-like specificity. Together with chymases and Cathepsin G, tryptases are important players in mast cell mediation of inflammatory and allergic responses. Tryptase alpha/beta-1(TPSAB1), also known as mast cell protease 7 (MCPT7), it exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of a diverse array of protease inhibitors in plasma. The two Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates. It may play a role in innate immunity.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 μg | In stock | $ 118.00 | |
50 μg | 5 days | $ 350.00 | |
500 μg | 5 days | $ 1,900.00 | |
1 mg | 5 days | $ 2,970.00 |
Biological Information |
Loaded Anti-Human Tryptase mAb-Fc on Protein A Biosensor, can bind Human TPSAB1-His with an affinity constant of 0.92 nM as determined in BLI assay. (Regularly tested)
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Description | Tryptases are serine proteases with trypsin-like specificity. Together with chymases and Cathepsin G, tryptases are important players in mast cell mediation of inflammatory and allergic responses. Tryptase alpha/beta-1(TPSAB1), also known as mast cell protease 7 (MCPT7), it exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of a diverse array of protease inhibitors in plasma. The two Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates. It may play a role in innate immunity. |
Species | Human |
Expression System | Human Cells |
Tag | C-6His |
Accession Number | Q86TM8 |
Synonyms | TPS1, tryptase alpha/beta 1, TPS2, tryptase α/β1, tryptase α/β 1, TPSB1, TPSAB1, Tryptase 1 |
Amino Acid | Ile31-Pro275 |
Construction | Recombinant Human Tryptase Alpha/Beta-1 is produced by our Mammalian expression system and the target gene encoding Ile31-Pro275 is expressed with a 6His tag at the C-terminus. |
Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Molecular Weight | 32-38 KDa, reducing conditions |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 1M NaCl, 0.05mM Heparin, 50mM sodium acetate, 0.01% sodium azide, pH 5.0. |
Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
Shipping |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Research Background | Tryptases are serine proteases with trypsin-like specificity. Together with chymases and Cathepsin G, tryptases are important players in mast cell mediation of inflammatory and allergic responses. Tryptase alpha/beta-1(TPSAB1), also known as mast cell protease 7 (MCPT7), it exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of a diverse array of protease inhibitors in plasma. The two Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates. It may play a role in innate immunity. |
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Please read the User Guide of Recombinant Proteins for more specific information.
TPSAB1 Protein, Human, Recombinant (His) TPS1 TPSAB 1 TPSB 1 TPS 2 TPS-1 TPS 1 tryptase alpha/beta 1 TPS2 tryptase α/β1 tryptase α/β 1 TPSB1 tryptase a/b 1 TPSAB1 Tryptase 1 TPS-2 TPSAB-1 TPSB-1 recombinant recombinant-proteins proteins protein