store at low temperature,keep away from moisture,keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Boc-Val-Pro-Arg-MCA hydrochloride (Boc-Val-Pro-Arg-MCA HCl) is a sensitive fluorescent substrate for the determination of trypsin-like serine proteases activity.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | Inquiry | $ 91.00 | |
5 mg | Inquiry | $ 266.00 | |
10 mg | Inquiry | $ 398.00 | |
25 mg | Inquiry | $ 717.00 | |
50 mg | Inquiry | $ 1,075.00 | |
100 mg | Inquiry | $ 1,613.00 |
Description | Boc-Val-Pro-Arg-MCA hydrochloride (Boc-Val-Pro-Arg-MCA HCl) is a sensitive fluorescent substrate for the determination of trypsin-like serine proteases activity. |
In vitro |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline and should be modified according to your specific needs). Add diluted Trypsin enzyme (100 μL) to 800 μL of 50mM Tris-HCl buffer (pH 8.0). Immediately initiate the reaction by adding 100 μL of 50 μM substrate Boc-Val-Pro-Arg-AMC hydrochloride. Incubate at 55°C for 10 minutes.Stop the reaction by adding 1.5 mL of stop solution (methanol: n-butanol: distilled water = 35:30:35, v/v/v). Measure fluorescence intensity using a fluorescence spectrophotometer (excitation 380 nm, emission 450 nm). |
Synonyms | Boc-Val-Pro-Arg-MCA HCl |
Molecular Weight | 664.19 |
Formula | C31H46ClN7O7 |
CAS No. | 70375-24-5 |
store at low temperature,keep away from moisture,keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Boc-Val-Pro-Arg-MCA hydrochloride 70375-24-5 Boc-Val-Pro-Arg-MCA HCl inhibitor inhibit