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Hank's Balanced Salt Solution (Ca2+, Mg2+, No phenol red)
🥰Excellent
Catalog No. TSH-00548
Alias HBSS
Hank's Balanced Salt Solution (Ca2+, Mg2+, No phenol red) is a classic isotonic buffer solution suitable for cell cleaning, pretreatment of trypsin digestion, and short-term storage and transfer of cells. This product supplements Ca2+ and Mg2+ on the basis of the classic HBSS formula, providing the basic ion environment and osmotic pressure support required by cells. The ingredients include 137.93 mM NaCl, 5.33 mM KCl, 4.17 mM NaHCO3, 0.441 mM KH2PO4, 0.338 mM Na2HPO4, 5.56 mM D-Glucose, 1.26 mM CaCl2, 0.493 mM MgCl2, 0.407 mM MgSO4, and is free of phenol red.
Hank's Balanced Salt Solution (Ca2+, Mg2+, No phenol red)
Hank's Balanced Salt Solution (Ca2+, Mg2+, No phenol red)
🥰Excellent
Catalog No. TSH-00548Alias HBSS
Hank's Balanced Salt Solution (Ca2+, Mg2+, No phenol red) is a classic isotonic buffer solution suitable for cell cleaning, pretreatment of trypsin digestion, and short-term storage and transfer of cells. This product supplements Ca2+ and Mg2+ on the basis of the classic HBSS formula, providing the basic ion environment and osmotic pressure support required by cells. The ingredients include 137.93 mM NaCl, 5.33 mM KCl, 4.17 mM NaHCO3, 0.441 mM KH2PO4, 0.338 mM Na2HPO4, 5.56 mM D-Glucose, 1.26 mM CaCl2, 0.493 mM MgCl2, 0.407 mM MgSO4, and is free of phenol red.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Hank's Balanced Salt Solution (Ca2+, Mg2+, No phenol red) is a classic isotonic buffer solution suitable for cell cleaning, pretreatment of trypsin digestion, and short-term storage and transfer of cells. This product supplements Ca2+ and Mg2+ on the basis of the classic HBSS formula, providing the basic ion environment and osmotic pressure support required by cells. The ingredients include 137.93 mM NaCl, 5.33 mM KCl, 4.17 mM NaHCO3, 0.441 mM KH2PO4, 0.338 mM Na2HPO4, 5.56 mM D-Glucose, 1.26 mM CaCl2, 0.493 mM MgCl2, 0.407 mM MgSO4, and is free of phenol red. |
| Synonyms | HBSS |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | 01 |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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