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Enterokinase Protein, Bovine, Recombinant (Animal-Free, His)
(Synonyms: PRSS7, ENTK, Enteropeptidase) Copy Product InfoSynonyms: PRSS7, ENTK, Enteropeptidase
Catalog No. TMPS-00026 Copy Product Info
Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.
This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions.
This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 100 U | $136 | 7-10 days | 7-10 days | |
| 500 U | $362 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 5 U/μl Unit definition: One unit is defined as the amount of enzyme sufficient to cleave 50 μg of control fusion protein (~24 kDa) in 16 hours to 95% completion at 22 °C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl 2 , pH 7.4. |
| Description | Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site. This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions. |
| Species | Bovine |
| Tag | His |
| Protein Purity | ≥ 95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of protein as determined by the LAL method. |
| Formulation | Supplied as solution in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50% glycerol, pH 7.4. |
| Stability & Storage | Upon receiving, the product remains stable for up to 6 months at -20 °C. This product is stable for up to 1 week at 37 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C. |
| Shipping | Proteins are shipped with blue ice. |
| Synonyms | PRSS7, ENTK, Enteropeptidase |
| Research Background |
| Molecular Weight | 40 kDa (Reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: Enterokinase Protein, Bovine, Recombinant (Animal-Free, His) chemical structure | Enterokinase Protein, Bovine, Recombinant (Animal-Free, His) molecular weight
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