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Enterokinase Protein, Bovine, Recombinant (His)
(Synonyms: PRSS7, ENTK, Enteropeptidase) Copy Product InfoSynonyms: PRSS7, ENTK, Enteropeptidase
Catalog No. TMPS-00025 Copy Product Info
Enterokinase (EK) is a duodenal protease involved in digestion by activating trypsinogen into trypsin, which in turn activates other pancreatic enzymes. EK specifically cleaves after the sequence Asp-Asp-Asp-Asp-Lys (DDDDK↓) and is inactive if a proline follows the site.
Recombinant bovine enterokinase (rbEK) shows higher activity than EK from other species and is widely used in biochemical applications. It is a glycosylated single-chain protein of ~200 amino acids, tagged with C-terminal 6×His for Ni²⁺ affinity purification and easy removal after digestion. The purified rbEK has a molecular weight of ~40 kDa and is fully biologically active.
Recombinant bovine enterokinase (rbEK) shows higher activity than EK from other species and is widely used in biochemical applications. It is a glycosylated single-chain protein of ~200 amino acids, tagged with C-terminal 6×His for Ni²⁺ affinity purification and easy removal after digestion. The purified rbEK has a molecular weight of ~40 kDa and is fully biologically active.
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| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $84 | 7-10 days | 7-10 days | |
| 10 μg | $136 | 7-10 days | 7-10 days | |
| 20 μg | $158 | 7-10 days | 7-10 days | |
| 50 μg | $198 | 7-10 days | 7-10 days | |
| 100 μg | $313 | 7-10 days | 7-10 days | |
| 200 μg | $492 | 7-10 days | 7-10 days | |
| 500 μg | $942 | 7-10 days | 7-10 days | |
| 1 mg | $1,520 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Enterokinase (EK) is a duodenal protease involved in digestion by activating trypsinogen into trypsin, which in turn activates other pancreatic enzymes. EK specifically cleaves after the sequence Asp-Asp-Asp-Asp-Lys (DDDDK↓) and is inactive if a proline follows the site. Recombinant bovine enterokinase (rbEK) shows higher activity than EK from other species and is widely used in biochemical applications. It is a glycosylated single-chain protein of ~200 amino acids, tagged with C-terminal 6×His for Ni²⁺ affinity purification and easy removal after digestion. The purified rbEK has a molecular weight of ~40 kDa and is fully biologically active. |
| Species | Bovine |
| Tag | His |
| Protein Purity | > 95% as determined by SDS-PAGE. |
| Endotoxin | <0.2 EU/μg, determined by LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2. |
| Reconstitution | Reconstitute lyophilized EK powder in sterile deionized water at 1mg/ml, then dilute in EK Dilution/Storage Buffer according to your requirement. |
| Stability & Storage | Lyophilized recombinant Bovine Enterokinase (His-tagged) (rbEK) remains stable up to one year at -20°C from date of receipt. Please avoid freeze-thaw cycles. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | PRSS7, ENTK, Enteropeptidase |
| Research Background |
| Molecular Weight | 40 kDa (Reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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You can also refer to dose conversion for different animals. More Dose Conversion
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: Enterokinase Protein, Bovine, Recombinant (His) chemical structure | Enterokinase Protein, Bovine, Recombinant (His) molecular weight
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