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Anti-Serpin A1 Antibody (8W352)
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Catalog No. TMAC-00150
Alias Serpin A1, Alpha-1-antitrypsin, Alpha-1-antiproteinase, Alpha-1 protease inhibitor
Anti-Serpin A1 Antibody (8W352) is a Rabbit antibody targeting Serpin A1. Anti-Serpin A1 Antibody (8W352) can be used in WB,ICC/IF,IHC,IP.
Anti-Serpin A1 Antibody (8W352)
Anti-Serpin A1 Antibody (8W352)
😃Good
Catalog No. TMAC-00150Alias Serpin A1, Alpha-1-antitrypsin, Alpha-1-antiproteinase, Alpha-1 protease inhibitor
Anti-Serpin A1 Antibody (8W352) is a Rabbit antibody targeting Serpin A1. Anti-Serpin A1 Antibody (8W352) can be used in WB,ICC/IF,IHC,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-Serpin A1 Antibody (8W352) is a Rabbit antibody targeting Serpin A1. Anti-Serpin A1 Antibody (8W352) can be used in WB,ICC/IF,IHC,IP. |
| Synonyms | Serpin A1, Alpha-1-antitrypsin, Alpha-1-antiproteinase, Alpha-1 protease inhibitor |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of alpha 1 Antitrypsin on human spleen lysates using anti-alpha 1 Antitrypsin antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin. 5. ICC staining alpha 1 Antitrypsin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining alpha 1 Antitrypsin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining alpha 1 Antitrypsin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cumulative damage to lung tissue by Neutrophil Elastase is responsible for the development of pulmonary emphysema, an irreversible lung disease characterized by loss of lung elasticity. a 1-antitrypsin (AAT), a 394 amino acid hepatic acute phase protein, predominantly inhibits Neutrophil Elastase. AAT is highly expressed in liver and in cultured hepatoma cells and, to a lesser extent, in macrophages. AAT is a highly polymorphic glycosylated serum protein with characteristic isoelectric-focusing patterns for most variants. The gene encoding AAT maps to a region of human chromosome 14 that includes a related serine protease inhibitor (serpin) gene which encodes corticosteroid-binding globulin. Oxidation of the methionine 358 residue in the active center of AAT results in a dramatic decrease in inhibitory activity towards elastase. AAT also has a moderate affinity for plasmin and Thrombin. AAT deficiency is associated with a 20-30 fold increased risk of precocious pulmonary emphysema. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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