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Anti-AACT Antibody (1F833) (Synonyms: SERPINA3, serpin family A member 3, Serpin A3, GIG25, GIG24, Cell gro...)
Catalog No. TMAB-02123 Copy Product Info
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Anti-AACT Antibody (1F833) is a Rabbit antibody targeting AACT. Anti-AACT Antibody (1F833) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-AACT Antibody (1F833)
Copy Product Info🥰Excellent
Catalog No. TMAB-02123
Synonyms SERPINA3, serpin family A member 3, Serpin A3, GIG25, GIG24, Cell growth-inhibiting gene 24/25 protein, alpha-1-antichymotrypsin, ACT, AACT_HUMAN
Anti-AACT Antibody (1F833) is a Rabbit antibody targeting AACT. Anti-AACT Antibody (1F833) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-AACT Antibody
(1F833)
(1F833)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-AACT Antibody (1F833) is a Rabbit antibody targeting AACT. Anti-AACT Antibody (1F833) can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | SERPINA3, serpin family A member 3, Serpin A3, GIG25, GIG24, Cell growth-inhibiting gene 24/25 protein, alpha-1-antichymotrypsin, ACT, AACT_HUMAN |
| Ig Type | IgG |
| Clone | 1F833 |
| Reactivity | Human |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with AACT monoclonal antibody, unconjugated (TMAB-02123) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AACT Monoclonal Antibody, Unconjugated (TMAB-02123) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | WB: 1:2000-10000; IHC-P: 1:200-800; IHC-Fr: 1:200-800; IF: 1:200-800 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Tissue Specificity | Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Lyophilized or Liquid |
| Formulation | PBS, Glycerol, BSA. |
| Concentration | 1mg/ml |
| Research Background | Alpha 1-Antichymotrypsin, a member of the serine proteinase inhibitor (serpin) family, inhibits neutrophil proteinase cathepsin G and mast cell chymases, and protects the lower respiratory tract from damage by proteolytic enzymes. It contains a reactive centre loop, which interacts with cognate proteinases, resulting in loop cleavage and a major conformational change. Recently, alpha 1-antichymotrypsin has been identified as a major constituent of the neurofibrillary plaques associated with Alzheimers disease, and in vitro studies have shown that it enhances the rate of amyloid-fibril formation. These observations and recent genetic evidence suggest that alpha 1-antichymotrypsin is important in the pathogenesis of Alzheimers disease. |
| Immunogen | Full length native Protein |
| Antigen Species | Human |
| Gene Name | SERPINA3 |
| Gene ID | |
| Protein Name | alpha-1-antichymotrypsin |
| Uniprot ID | |
| Function | Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. |
| Molecular Weight | Theoretical: 45 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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