mrn

(Z)-Mirin is a potent Mre11–Rad50–Nbs1 (MRN) complex inhibitor, and inhibits Mre11-associated exonuclease activity.

eGFP mRNA-LNP (500 ng/uL) is a lipid nanoparticle (LNP) containing eGFP mRNA, suitable for assays of RNA delivery, translation efficiency, and cell viability. eGFP circRNA includes the Enhanced Green Fluorescent Protein (eGFP), which expresses green fluorescent protein upon entering cells. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry.

HumanNLRP3mRNA encodes the NLRP3 protein, which functions as an inflammasome. NLRP3 is part of the NLR protein family and plays a critical role in immune responses and human diseases.

BCMA CARmRNA encodes a CAR protein targeting human BCMA and induces temporary CAR expression in T cells without the necessity for permanent genetic modification. It is used in cancer research as it targets BCMA, a member of the tumor necrosis factor receptor superfamily 17 (TNFRSF17), also known as B-cell maturation antigen (CD269). This receptor supports B-cell survival and plays a role in regulating humoral immunity. Notably, BCMA is highly expressed in multiple myeloma and serves as a biomarker for its diagnosis.

The COVID-19 Spike Protein mRNA, responsible for expressing the COVID-19 spike protein, is crucial for evaluating RNA delivery, translation efficiency, and cell viability. Positioned on the membrane surface, this spike protein facilitates virus entry into cells by binding to host cell membrane receptors and promoting membrane fusion. It serves as a significant action site for neutralizing antibodies and represents a primary focus in vaccine development [1].

CD19 CAR mRNA is designed to express the CD19 chimeric antigen receptor (CAR) protein, pivotal in chimeric antigen receptor T cell (CAR-CD19) immunotherapy. This receptor targets CD19, a membrane antigen crucial for B cell proliferation, differentiation, activation, and antibody synthesis, playing a significant role in promoting BCR signal transduction.

CD19 car mRNA-LNP, a lipid nanoparticle (LNP) encapsulating CD19 car mRNA, is instrumental in assessing RNA delivery, translation efficacy, and cell viability among other applications. This compound is pivotal for chimeric antigen receptor T cell immunotherapy (CAR-CD19), leveraging the CD19 molecule - a critical membrane antigen expressed by B cells (i.e., leukocyte differentiation antigen) that plays a significant role in their proliferation, differentiation, activation, and antibody production, as well as enhancing BCR signal transduction [1] [2].

COVID-19 Spike Protein mRNA-LNP is a lipid nanoparticle (LNP) formulated with the novel coronavirus pneumonia spike protein, positioned on the membrane surface. It is designed for applications such as RNA delivery, translation efficiency, and cell viability assessment. This spike protein facilitates the COVID-19 virus's entry into host cells by binding to cell membrane receptors and enabling membrane fusion, making it a critical focal point for host neutralizing antibodies and vaccine development [1].

Firefly Luciferase mRNA (N1-Me-Pseudo UTP) is an mRNA sequence encoding firefly luciferase, which can express luciferase protein in cells after transfection, and is commonly used in gene regulation, cell viability assay, ATP level analysis and in vivo imaging, etc. It can be used as a positive control for mRNA transfection, and yellow-green fluorescence is observed at 560 nm.

eGFP mRNA-LNP is a lipid nanoparticle encapsulating eGFP mRNA, designed to evaluate RNA delivery, translation efficiency, and cell viability. Upon cell internalization, it expresses Enhanced Green Fluorescent Protein (eGFP), enabling detection through fluorescence microscopy or flow cytometry [1].

eGFP mRNA encodes the green fluorescent protein, originally derived from the jellyfish Aequorea victoria, and serves as a widely utilized direct detection reporter in mammalian cell culture. This protein produces a distinct bright green fluorescence, with an emission peak at 509 nm.

mRNA-Encapsulating Lipid Excipient 1 (Compound 2), a thiocarbamate-based ionizable lipid, serves as an excipient in the formulation of mRNA-encapsulating lipid nanoparticles [1].

eGFP(cy5) mRNA is a chemically modified synthetic mRNA designed for high-sensitivity tracking of gene delivery processes. The compound features a dual-functional design: its nucleotide sequence encodes the enhanced Green Fluorescent Protein (eGFP) to quantify translation efficiency, while the mRNA backbone is covalently labeled with the near-infrared fluorophore Cy5. This dual-labeling system enables researchers to distinguish the physical distribution of mRNA (uptake/endocytosis, visualized as Cy5 red signal) from its functional biological activity (protein expression, visualized as eGFP green signal) at the single-cell level. It is widely utilized in lipid nanoparticle (LNP) formulation optimization, endocytic pathway elucidation, and tissue distribution studies.

Cas12amRNA encodes the Cas12a protein, which functions as an endonuclease.

mRNACap 2'-O-methyltransferase uses S-adenosylmethionine (SAM) as a methyl donor to add a methyl group at the 2'-O position of the first nucleotide at the 5' end of Cap-0 mRNA, resulting in the formation of a Cap-1 structure. This Cap-1 structure enhances translation efficiency and boosts subsequent protein expression.

β-galactosidase mRNA (N1-Methylpseudo-UTP) encodes the bacterial lactose Z gene product, β-galactosidase. This enzyme catalyzes the conversion of β-galactosides into monosaccharides and serves as a universal marker for assessing transfection efficiency. The inclusion of N1-Methylpseudo-UTP reduces the immunogenicity of the generated mRNA.

Toyocamycin (Vengicide) is an adenosine analog produced by Actinomycetes, functioning as an XBP1 inhibitor and inhibiting IRE1α-induced ATP-dependent XBP1 mRNA cleavage (IC50: 80 nM), and induces apoptosis.

SM-102 is the only ionizable amino cationic lipid currently clinically approved for RNA therapeutics and can be used to synthesize lipid nanoparticles (LNPs). It shows potential in the development of LNPs for the delivery of mRNA-based vaccines.

Temarotene (Ro 15-0778) is an orally active and potent small molecule compound that modulates human immune function in vitro.Temarotene may be used in the study of lichen planus.

Budesonide (Pulmicort), an anti-inflammatory corticosteroid, has shown the effective glucocorticoid activitie and few mineralocorticoid activities. According to reports, Budesonide has extensively inhibitory effects against multiple cells types and mediators referred to allergic and nonallergic-mediated inflammatory. What's more, the anti-inflammatory action of budesonide has been revealed to contribute to the effectiveness of asthma.

Enoxacin hydrate (AT-2266 hydrate) is a broad-spectrum 6-fluoronaphthyridinone antibacterial agent.

Enoxacin (NSC-629661) is a broad-spectrum 6-fluoronaphthyridinone antibacterial agent (fluoroquinolones) structurally related to nalidixic acid.

Ceramides Mixture, an endogenous ceramide, consists of hydroxy and non-hydroxy fatty acid-containing ceramides. It is involved in the regulation of cell cycle arrest, growth inhibition, and modulation of telomerase activity.

Taurochenodeoxycholic Acid (12-Deoxycholyltaurine) is one of the main bioactive substances of animals' bile acid.