Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-PRPF8 Antibody (4V102)
😃Good
Catalog No. TMAC-03447
Alias U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p, U5 snRNP specific protein, Splicing factor Prp8, SNRNP220, RP13, Retinitis pigmentosa 13 (autosomal dominant), Prpf 8, PRPC8, PRP8_HUMAN, PRP8 pre mRNA processing factor 8 homolog, PRP8 homolog, PRP8, Pre-mRNA-processing-splicing factor 8, Precursor mRNA processing protein, Pre mRNA-processing factor 8, S. cerevisiae, homolog of, Pre mRNA processing factor 8, p220, HPRP8, Apoptosis regulated protein 2, Apoptosis regulated protein 1, 220 kDa U5 snRNP-specific protein, 220 kDa U5 snRNP specific protein
Anti-PRPF8 Antibody (4V102) is a Rabbit antibody targeting PRPF8. Anti-PRPF8 Antibody (4V102) can be used in WB,ICC,IHC,FCM.
Anti-PRPF8 Antibody (4V102)
Anti-PRPF8 Antibody (4V102)
😃Good
Catalog No. TMAC-03447Alias U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p, U5 snRNP specific protein, Splicing factor Prp8, SNRNP220, RP13, Retinitis pigmentosa 13 (autosomal dominant), Prpf 8, PRPC8, PRP8_HUMAN, PRP8 pre mRNA processing factor 8 homolog, PRP8 homolog, PRP8, Pre-mRNA-processing-splicing factor 8, Precursor mRNA processing protein, Pre mRNA-processing factor 8, S. cerevisiae, homolog of, Pre mRNA processing factor 8, p220, HPRP8, Apoptosis regulated protein 2, Apoptosis regulated protein 1, 220 kDa U5 snRNP-specific protein, 220 kDa U5 snRNP specific protein
Anti-PRPF8 Antibody (4V102) is a Rabbit antibody targeting PRPF8. Anti-PRPF8 Antibody (4V102) can be used in WB,ICC,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
Add to Cart
Add to Quotation
Bulk & Custom
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View MoreResource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PRPF8 Antibody (4V102) is a Rabbit antibody targeting PRPF8. Anti-PRPF8 Antibody (4V102) can be used in WB,ICC,IHC,FCM. |
| Synonyms | U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p, U5 snRNP specific protein, Splicing factor Prp8, SNRNP220, RP13, Retinitis pigmentosa 13 (autosomal dominant), Prpf 8, PRPC8, PRP8_HUMAN, PRP8 pre mRNA processing factor 8 homolog, PRP8 homolog, PRP8, Pre-mRNA-processing-splicing factor 8, Precursor mRNA processing protein, Pre mRNA-processing factor 8, S. cerevisiae, homolog of, Pre mRNA processing factor 8, p220, HPRP8, Apoptosis regulated protein 2, Apoptosis regulated protein 1, 220 kDa U5 snRNP-specific protein, 220 kDa U5 snRNP specific protein |
| Ig Type | IgG |
| Clone | 4V102 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PRPF8 on SiHa (1) and A549 (2) cell lysate using anti-PRPF8 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PRPF8 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PRPF8 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-PRPF8 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PRPF8 antibody. Counter stained with hematoxylin. 6. ICC staining PRPF8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining PRPF8 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining PRPF8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of A549 cells with PRPF8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | PRP8, also designated pre-mRNA-processing-splicing factor 8, is a highly conserved nuclear protein and a central component of the catalytic core of the spliceosome, where it may be involved in various molecular rearrangements. PRP8, which is widely expressed, plays a role in transesterification reactions that regulate splicesome-induced pre-mRNA splicing. Specifically, PRP8 interacts with the GU dinucleotide at the 5' splice site (5'SS) and forms a specific UV-inducible cross-link. It also interacts functionally with the 3'SS, affecting the efficiency of the second catalytic step. PRP8 may play a role in the first transesterification step, as PRP8 mutations that prohibit negative regulation of PRP28 or PRP44/Brr2 subsequently block U4 activation. In addition, PRP8 interacts with a conserved region of U6 that is instrumental in the formation of the catalytic core of the spliceosome. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: 1C200 of human PRPF8 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 274 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Sci Citations
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-PRPF8 Antibody (4V102) | purchase Anti-PRPF8 Antibody (4V102) | Anti-PRPF8 Antibody (4V102) cost | order Anti-PRPF8 Antibody (4V102) | Anti-PRPF8 Antibody (4V102) molecular weight
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.