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Anti-FTO Polyclonal Antibody
😃Good
Catalog No. TMAB-00719
Anti-FTO Polyclonal Antibody is a Rabbit antibody targeting FTO. Anti-FTO Polyclonal Antibody can be used in WB.
Anti-FTO Polyclonal Antibody
Anti-FTO Polyclonal Antibody
😃Good
Catalog No. TMAB-00719
Anti-FTO Polyclonal Antibody is a Rabbit antibody targeting FTO. Anti-FTO Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-FTO Polyclonal Antibody is a Rabbit antibody targeting FTO. Anti-FTO Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | Sample: Lane 1: Mouse Adrenal gland tissue lysates Lane 2: Rat Adrenal gland tissue lysates Lane 3: Human SH-SY5Y cell lysates Lane 4: Human HeLa cell lysates Lane 5: Human U87MG cell lysates Lane 6: Human MOLT4 cell lysates Primary: Anti-FTO (TMAB-00719) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kDa Observed band size: 60 kDa  |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. |
| Immunogen | KLH conjugated synthetic peptide: human Protein fatso |
| Antigen Species | Human |
| Gene Name | FTO |
| Gene ID | |
| Protein Name | alpha-ketoglutarate-dependent dioxygenase FTO |
| Uniprot ID | |
| Biology Area | Diabetes associated,Lipid transport,Lipoprotein metabolism,Lipoprotein metabolism,Cancer,Diabetes,Heart disease,Metabolic disorders,Obesity,Metabolism |
| Function | Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. |
| Molecular Weight | Theoretical: 58 kDa. Actual: 58 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
α-ketoglutarate-dependent dioxygenase FTOU6 small nuclear RNA N(6)-methyladenosine-demethylase FTOU6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTOtRNA N1-methyl adenine demethylase FTOmRNA N(6)-methyladenosine demethylase FTOmRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO (m6A(m)-demethylase FTO)m6A(m-demethylase FTO)KIAA1752KIAA 1752FTOFat mass and obesity-associated proteinAlpha-ketoglutarate-dependent dioxygenase FTOa-ketoglutarate-dependent dioxygenase FTO
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