Name: eGFP(cy5) mRNA
Brand: TargetMol
SKU: TMRH-0018
Rating: 4.5 (1 reviews)

eGFP(cy5) mRNA

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Catalog No. TMRH-0018

eGFP(cy5) mRNA is a chemically modified synthetic mRNA designed for high-sensitivity tracking of gene delivery processes. The compound features a dual-functional design: its nucleotide sequence encodes the enhanced Green Fluorescent Protein (eGFP) to quantify translation efficiency, while the mRNA backbone is covalently labeled with the near-infrared fluorophore Cy5. This dual-labeling system enables researchers to distinguish the physical distribution of mRNA (uptake/endocytosis, visualized as Cy5 red signal) from its functional biological activity (protein expression, visualized as eGFP green signal) at the single-cell level. It is widely utilized in lipid nanoparticle (LNP) formulation optimization, endocytic pathway elucidation, and tissue distribution studies.

eGFP(cy5) mRNA

eGFP(cy5) mRNA

😃Good

Catalog No. TMRH-0018

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
100 μg	Inquiry	Inquiry	Inquiry
100 μg * 10	Inquiry	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Product Introduction

Bioactivity

Description	eGFP(cy5) mRNA is a chemically modified synthetic mRNA designed for high-sensitivity tracking of gene delivery processes. The compound features a dual-functional design: its nucleotide sequence encodes the enhanced Green Fluorescent Protein (eGFP) to quantify translation efficiency, while the mRNA backbone is covalently labeled with the near-infrared fluorophore Cy5. This dual-labeling system enables researchers to distinguish the physical distribution of mRNA (uptake/endocytosis, visualized as Cy5 red signal) from its functional biological activity (protein expression, visualized as eGFP green signal) at the single-cell level. It is widely utilized in lipid nanoparticle (LNP) formulation optimization, endocytic pathway elucidation, and tissue distribution studies.
In vitro	In cancer cell models (e.g., A549, HepG2), eGFP(cy5) mRNA delivered via LNPs allowed for the profiling of endocytic escape; flow cytometry and confocal microscopy revealed that while over 90% of cells were Cy5-positive (indicating successful uptake), only a fraction showed eGFP expression, highlighting the "lost in translation" phenomenon during intracellular trafficking [1][3].
In vivo	In mouse models of lung fibrosis and liver distribution, systemic or local administration of LNP-encapsulated eGFP(cy5) mRNA demonstrated robust accumulation in liver macrophages and lung tissues; Cy5 signals confirmed organ-specific localization, while eGFP fluorescence quantified the extent of functional protein synthesis in different cell types [1][2].
Research Background	Upon transfection into cells, eGFP mRNA enables intracellular expression of enhanced green fluorescent protein (eGFP). The wild-type protein (GFP) was originally discovered in 1962 by Osamu Shimomura from the jellyfish Aequorea victoria. GFP exhibits unique auto-fluorescent properties that require no cofactors or substrates beyond molecular oxygen. Consequently, GFP is frequently fused to proteins of interest to monitor their expression and localization. However, GFP has significant limitations in practical applications. To address these, molecular biologists developed an engineered variant termed eGFP, which maintains identical secondary structure to GFP. Crucially, eGFP incorporates codon optimization for enhanced expression in mammalian cells.

Chemical Properties

Storage & Solubility Information

Storage

store at low temperature | In solvent: -80°C for 1 year

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

eGFP(cy5) mRNA

eGFP(cy5) mRNA

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In Vivo Formulation Calculator (Clear solution)

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