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Anti-RPRD1B Antibody (8O830)

😃Good
Catalog No. TMAY-02684
Alias regulation of nuclear pre-mRNA domain containing 1B, NET60, dJ1057B20.2, CREPT, C20orf77

Anti-RPRD1B Antibody (8O830) is a Mouse antibody targeting RPRD1B. Anti-RPRD1B Antibody (8O830) can be used in WB.

Anti-RPRD1B Antibody (8O830)

Anti-RPRD1B Antibody (8O830)

😃Good
Catalog No. TMAY-02684Alias regulation of nuclear pre-mRNA domain containing 1B, NET60, dJ1057B20.2, CREPT, C20orf77
Anti-RPRD1B Antibody (8O830) is a Mouse antibody targeting RPRD1B. Anti-RPRD1B Antibody (8O830) can be used in WB.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-RPRD1B Antibody (8O830) is a Mouse antibody targeting RPRD1B. Anti-RPRD1B Antibody (8O830) can be used in WB.
Aliasregulation of nuclear pre-mRNA domain containing 1B, NET60, dJ1057B20.2, CREPT, C20orf77
Ig Type
Monoclonal Mouse IgG2b
Clone
8O830
Reactivity
Human
Verified Activity
Anti-RPRD1B mouse monoclonal antibody at 1:500 dilution.

-Lane A: Jurkat Whole Cell Lysate.

-Lane B: Hela Whole Cell Lysate.

-Lane C: HepG2 Whole Cell Lysate.

-Lane D: 293T Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:37 kDa.

-Observed band size:37 kDa
Application
WB
Recommended Dose
WB: 1:500-1:2000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RPRD1B (rh RPRD1B; TMPY-03599; AAH33629.1; Met1-Asp326). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundRPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator that enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human RPRD1B Protein (TMPY-03599)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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