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Anti-RPRD1B Antibody (8O830) is a Mouse antibody targeting RPRD1B. Anti-RPRD1B Antibody (8O830) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $198 | 7-10 days |
Description | Anti-RPRD1B Antibody (8O830) is a Mouse antibody targeting RPRD1B. Anti-RPRD1B Antibody (8O830) can be used in WB. |
Alias | regulation of nuclear pre-mRNA domain containing 1B, NET60, dJ1057B20.2, CREPT, C20orf77 |
Ig Type | Monoclonal Mouse IgG2b |
Clone | 8O830 |
Reactivity | Human |
Verified Activity | Anti-RPRD1B mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: Hela Whole Cell Lysate. -Lane C: HepG2 Whole Cell Lysate. -Lane D: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:37 kDa. -Observed band size:37 kDa |
Application | WB |
Recommended Dose | WB: 1:500-1:2000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RPRD1B (rh RPRD1B; TMPY-03599; AAH33629.1; Met1-Asp326). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | RPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator that enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human RPRD1B Protein (TMPY-03599) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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