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Anti-hUPF1 Antibody (4P615)
😃Good
Catalog No. TMAC-02076
Alias Yeast Upf1p homolog, Up-frameshift suppressor 1 homolog, UPF1 RNA helicase and ATPase, UPF1 regulator of nonsense transcripts homolog, upf1, UPF 1 regulator of nonsense transcripts homolog, UPF 1, Up frameshift suppressor 1 homolog, Up frameshift mutation 1 homolog (S. cerevisiae), Up frameshift mutation 1 homolog, UP Frameshift 1, Smg 2 homolog nonsense mediated mRNA decay factor, Smg 2, RENT1_HUMAN, RENT1, RENT 1, Regulator of nonsense transcripts 1, pNORF1, pNORF 1, NORF1, NORF 1, Nonsense mRNA reducing factor 1, KIAA0221, hUpf1, HUPF 1, hUPF 1, FLJ46894, FLJ43809, Delta helicase, ATP-dependent helicase RENT1, ATP dependent helicase RENT1
Anti-hUPF1 Antibody (4P615) is a Rabbit antibody targeting hUPF1. Anti-hUPF1 Antibody (4P615) can be used in WB,ICC,IF,IP,IHC,FCM.
Anti-hUPF1 Antibody (4P615)
Anti-hUPF1 Antibody (4P615)
😃Good
Catalog No. TMAC-02076Alias Yeast Upf1p homolog, Up-frameshift suppressor 1 homolog, UPF1 RNA helicase and ATPase, UPF1 regulator of nonsense transcripts homolog, upf1, UPF 1 regulator of nonsense transcripts homolog, UPF 1, Up frameshift suppressor 1 homolog, Up frameshift mutation 1 homolog (S. cerevisiae), Up frameshift mutation 1 homolog, UP Frameshift 1, Smg 2 homolog nonsense mediated mRNA decay factor, Smg 2, RENT1_HUMAN, RENT1, RENT 1, Regulator of nonsense transcripts 1, pNORF1, pNORF 1, NORF1, NORF 1, Nonsense mRNA reducing factor 1, KIAA0221, hUpf1, HUPF 1, hUPF 1, FLJ46894, FLJ43809, Delta helicase, ATP-dependent helicase RENT1, ATP dependent helicase RENT1
Anti-hUPF1 Antibody (4P615) is a Rabbit antibody targeting hUPF1. Anti-hUPF1 Antibody (4P615) can be used in WB,ICC,IF,IP,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-hUPF1 Antibody (4P615) is a Rabbit antibody targeting hUPF1. Anti-hUPF1 Antibody (4P615) can be used in WB,ICC,IF,IP,IHC,FCM. |
| Synonyms | Yeast Upf1p homolog, Up-frameshift suppressor 1 homolog, UPF1 RNA helicase and ATPase, UPF1 regulator of nonsense transcripts homolog, upf1, UPF 1 regulator of nonsense transcripts homolog, UPF 1, Up frameshift suppressor 1 homolog, Up frameshift mutation 1 homolog (S. cerevisiae), Up frameshift mutation 1 homolog, UP Frameshift 1, Smg 2 homolog nonsense mediated mRNA decay factor, Smg 2, RENT1_HUMAN, RENT1, RENT 1, Regulator of nonsense transcripts 1, pNORF1, pNORF 1, NORF1, NORF 1, Nonsense mRNA reducing factor 1, KIAA0221, hUpf1, HUPF 1, hUPF 1, FLJ46894, FLJ43809, Delta helicase, ATP-dependent helicase RENT1, ATP dependent helicase RENT1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- hUPF1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-hUPF1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- hUPF1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- hUPF1 antibody. Counter stained with hematoxylin. 6. ICC staining hUPF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining hUPF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining hUPF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC:IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability. Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5' to 3' helicase activity. In addition, Rent1 is an RNA-binding protein whose activity is modulated by ATP and directly interacts with Rent2, which is a mammalian homolog of Upf2p. Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by two separate genes. Rent3b (also known as Rent3X) is encoded by a X-linked gene and localizes primarily to the nucleus, while Rent 1 and Rent 2 localize primarily in the cytoplasm. Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human hUPF1 aa 1-200 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 124 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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