FTO Protein, Human, Recombinant (His & Myc)

Name: FTO Protein, Human, Recombinant (His & Myc)
Brand: TargetMol
SKU: TMPH-00920
Price: 89 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, tRNA N1-methyl adenine demethylase FTO, mRNA N(6)-methyladenosine demethylase FTO, mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO (m6A(m)-demethylase FTO), KIAA1752, FTO, Fat mass and obesity-associated protein, Alpha-ketoglutarate-dependent dioxygenase FTO) Copy Product Info

Synonyms: U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, tRNA N1-methyl adenine demethylase FTO, mRNA N(6)-methyladenosine demethylase FTO, mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO (m6A(m)-demethylase FTO), KIAA1752, FTO, Fat mass and obesity-associated protein, Alpha-ketoglutarate-dependent dioxygenase FTO

Catalog No. TMPH-00920 Copy Product Info

FTO Protein, Human, Recombinant (His & Myc) is expressed in E. coli.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$89	-	In Stock
10 µg	$143	-	In Stock
20 µg	$237	-	In Stock
50 µg	$358	20 days	20 days
100 µg	$490	20 days	20 days
200 µg	$755	20 days	20 days
500 µg	$1,330	20 days	20 days
1 mg	$2,080	20 days	20 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:91%

Appearance:Lyophilized powder

Color:White

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COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	FTO Protein, Human, Recombinant (His & Myc) is expressed in E. coli.
Species	Human
Expression System	E. coli
Tag	N-10xHis, C-Myc
Accession Number	Q9C0B1
Amino Acid	MKRTPTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIETIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTGTLDYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRYRKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLRREWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP
Construction	1-505 aa
Protein Purity	> 85% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, tRNA N1-methyl adenine demethylase FTO, mRNA N(6)-methyladenosine demethylase FTO, mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO (m6A(m)-demethylase FTO), KIAA1752, FTO, Fat mass and obesity-associated protein, Alpha-ketoglutarate-dependent dioxygenase FTO
Research Background	RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping. Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Ability to repair alkylated DNA and RNA is however unsure in vivo. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs. Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression.

Chemical Properties

Molecular Weight

65.7 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.