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Anti-RPRD1B Antibody (6Y357)
😃Good
Catalog No. TMAY-02686
Alias regulation of nuclear pre-mRNA domain containing 1B, NET60, dJ1057B20.2, CREPT, C20orf77
Anti-RPRD1B Antibody (6Y357) is a Mouse antibody targeting RPRD1B. Anti-RPRD1B Antibody (6Y357) can be used in ICC/IF.
Anti-RPRD1B Antibody (6Y357)
Anti-RPRD1B Antibody (6Y357)
😃Good
Catalog No. TMAY-02686Alias regulation of nuclear pre-mRNA domain containing 1B, NET60, dJ1057B20.2, CREPT, C20orf77
Anti-RPRD1B Antibody (6Y357) is a Mouse antibody targeting RPRD1B. Anti-RPRD1B Antibody (6Y357) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-RPRD1B Antibody (6Y357) is a Mouse antibody targeting RPRD1B. Anti-RPRD1B Antibody (6Y357) can be used in ICC/IF. |
| Alias | regulation of nuclear pre-mRNA domain containing 1B, NET60, dJ1057B20.2, CREPT, C20orf77 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 6Y357 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of RPRD1B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human RPRD1B monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Nucleus. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RPRD1B (rh RPRD1B; TMPY-03599; AAH33629.1; Met1-Asp326). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | RPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator that enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human RPRD1B Protein (TMPY-03599) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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