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Anti-Phospho-EIF4E (Ser209) Antibody (4B622)
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Catalog No. TMAC-03211
Alias Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19
Anti-Phospho-EIF4E (S209) Antibody (4B622) is a Rabbit antibody targeting Phospho-EIF4E (S209). Anti-Phospho-EIF4E (S209) Antibody (4B622) can be used in WB,ICC,IHC,IP.
Anti-Phospho-EIF4E (Ser209) Antibody (4B622)
Anti-Phospho-EIF4E (Ser209) Antibody (4B622)
😃Good
Catalog No. TMAC-03211Alias Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19
Anti-Phospho-EIF4E (S209) Antibody (4B622) is a Rabbit antibody targeting Phospho-EIF4E (S209). Anti-Phospho-EIF4E (S209) Antibody (4B622) can be used in WB,ICC,IHC,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-Phospho-EIF4E (Ser209) Antibody (4B622) is a Rabbit antibody targeting Phospho-EIF4E (Ser209). Anti-Phospho-EIF4E (Ser209) Antibody (4B622) can be used in WB,ICC,IHC,IP. |
| Synonyms | Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Phospho-eIF4E (S209) on different lysates using anti-Phospho-eIF4E (S209) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse spleen, Lane 2: Rat spleen. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin. 5. ICC staining Phospho-eIF4E (S209) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Phospho-eIF4E (S209) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Phospho-eIF4E (S209) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human eIF4E around the phosphorylation site of Ser209 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 25 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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