proteases

Fluorescent Substrate for Glu-Specific Proteases is a chromogenic substrate specifically recognized by V8 protease [1].

Fluorescent Substrate for Asp-Specific Proteases selectively cleaves the Asp-Val bond [1].

DQ-BSA-Red is a bovine serum albumin (BSA) molecule labeled with a red fluorescent dye, designed for monitoring lysosomal activity. The excitation and emission wavelengths of DQ-BSA-Red are 590 nm and 620 nm, respectively. The BSA in DQ-BSA-Red is heavily tagged with the red fluorescent dye at multiple sites, leading to significant fluorescence self-quenching. Upon entry into the lysosome, DQ-BSA is cleaved by lysosomal proteases, resulting in the unquenching and release of fluorescent fragments that emit bright fluorescence. Conversely, functionally inactive lysosomes fail to degrade the BSA protein, thereby displaying lower or no fluorescence signal.

Boc-Val-Pro-Arg-MCA hydrochloride (Boc-Val-Pro-Arg-MCA HCl) is a sensitive fluorescent substrate for the determination of trypsin-like serine proteases activity.

Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a substrate chromogen that releases a yellow 4-nitroaniline upon cleavage and is a substrate for many proteases and prostate-specific antigens (PAs), and can be used to identify novel protein hydrolytic activities in yeast mutants.

N-Acetyl-DL-phenylalanine β-naphthyl ester is a chromogenic substrate for chymotrypsin and microbial serine proteases like subtilisin [1]. This aromatic amino acid ester exhibits color changes upon enzymatic cleavage, facilitating the monitoring of enzyme activity.

BMV109 is a quenched probe designed to emit fluorescence upon being cleaved and covalently bound by active cathepsin proteases. This property of BMV109 is utilized in tumor imaging [1] [2].

Boc-Leu-Ser-Thr-Arg-AMC serves as a fluorogenic substrate linked with AMC for proteases [1].

Z-Gly-Pro-Arg-pNA (z-GPR-pNA) serves as a chromogenic substrate primarily used for the detection of prostate-specific antigen (PSA) activating proteases. Additionally, it can be utilized in the assessment of trypsin activity.