BID Protein, Mouse, Recombinant (His)

Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 534.00
500 μg	5 days	$ 2,140.00
1 mg	5 days	$ 3,570.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 534.00

500 μg

5 days

$ 2,140.00

1 mg

5 days

$ 3,570.00

Description

Species	Mouse
Expression System	E. coli
Tag	C-His
Accession Number	EDK99650.1
Synonyms	BID, p22 BID
Construction	Recombinant Mouse BID Protein is expressed from E.coli with His tag at the C-Terminus. It contains Ala1-Ser195.[Accession \|EDK99650.1]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 22.72 kDa same as Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.