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L-Leucyl-4-nitroanilide hydrochloride
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Catalog No. TXB-00290Cas No. 16010-98-3
L-Leucyl-4-nitroanilide hydrochloride is a substrate commonly employed in various biochemical assays for assessing the activity of enzymes such as proteases that cleave peptide bonds. With its unique chemical properties, L-Leucine p-nitroanilide hydrochloride can be cleaved by these enzymes to produce a yellow product known as p-nitroaniline. This characteristic makes it a valuable tool for detecting and quantifying protease activity in biological samples such as blood or tissue extracts.
L-Leucyl-4-nitroanilide hydrochloride
😃Good
Catalog No. TXB-00290Cas No. 16010-98-3
L-Leucyl-4-nitroanilide hydrochloride is a substrate commonly employed in various biochemical assays for assessing the activity of enzymes such as proteases that cleave peptide bonds. With its unique chemical properties, L-Leucine p-nitroanilide hydrochloride can be cleaved by these enzymes to produce a yellow product known as p-nitroaniline. This characteristic makes it a valuable tool for detecting and quantifying protease activity in biological samples such as blood or tissue extracts.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 10 mg | Inquiry | 7-10 days | |
| 50 mg | Inquiry | 7-10 days |
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Product Introduction
Bioactivity
Chemical Properties
| Description | L-Leucyl-4-nitroanilide hydrochloride is a substrate commonly employed in various biochemical assays for assessing the activity of enzymes such as proteases that cleave peptide bonds. With its unique chemical properties, L-Leucine p-nitroanilide hydrochloride can be cleaved by these enzymes to produce a yellow product known as p-nitroaniline. This characteristic makes it a valuable tool for detecting and quantifying protease activity in biological samples such as blood or tissue extracts. |
| Molecular Weight | 287.74 |
| Formula | C12H18ClN3O3 |
| Cas No. | 16010-98-3 |
Storage & Solubility Information
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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