The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 487.00 | |
500 μg | 5 days | $ 1,950.00 | |
1 mg | 5 days | $ 3,250.00 |
Description | The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. |
Species | SARS |
Expression System | E. coli |
Tag | C-His |
Accession Number | AAX16193.1 |
Synonyms | Papain-like Protease, PL-PRO, Replicase polyprotein 1a, pp1a, PL2-PRO, nsp1, Plpro |
Construction | Recombinant SARS PLpro/papain-like protease Protein is expressed from E.coli with His tag at the C-terminus. It contains Glu1541-Tyr1859.[Accession |AAX16193.1] |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | The protein has a predicted MW of 36.91 kDa same as Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Supplied as 0.22 μm filtered solution in 50 mM Tris, 0.1 % Tween, 150mM NaCl (pH 9.0). |
Stability & Storage |
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. |
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Please read the User Guide of Recombinant Proteins for more specific information.
SARS PLpro/papain-like protease Protein (His) Papain-like Protease PL-PRO Replicase polyprotein 1a pp1a PL2-PRO nsp1 Plpro recombinant recombinant-proteins proteins protein