SARS-CoV-2 PLpro/papain-like protease Protein (His & Avi)

The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 231.00
500 μg	5 days	$ 932.00
1 mg	5 days	$ 1,550.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 231.00

500 μg

5 days

$ 932.00

1 mg

5 days

$ 1,550.00

Description

Species	SARS
Expression System	E. coli
Tag	N-His-Avi
Accession Number	P0C6U8
Synonyms	Plpro, PL2-PRO, pp1a, nsp1, Replicase polyprotein 1a, Papain-like Protease, PL-PRO
Construction	Recombinant SARS-CoV-2 PLpro/papain-like protease Protein is expressed from E.coil with His tag and Avi tag at the N-Terminus. It contains Glu1-Ile314.[Accession \|P0C6U8]
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	The protein has a predicted MW of 38.6 kDa same as Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Supplied as 0.22μm filtered solution in 20mM Tris, 100mM NaCl, 10% Glycerol (pH 7.8).
Stability & Storage	Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses.